The PP6 Phosphatase Regulates ABI5 Phosphorylation and Abscisic Acid Signaling in Arabidopsis

doi:10.1105/tpc.112.105767

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	The PP6 Phosphatase Regulates ABI5 Phosphorylation and Abscisic Acid Signaling in Arabidopsis
	Dai, Mingqiu 1; Xue, Qin 1; Mccray, Tyra 1; Margavage, Kathryn 1,2; Chen, Fang 1; Lee, Jae-Hoon 1,3; Nezames, Cynthia D.1; Guo, Liquan 1,4; Terzaghi, William 1,2; Wan, Jianmin 5
刊名	PLANT CELL
	2013
卷号	25 期号:2 页码:517-534
ISSN号	1040-4651
DOI	10.1105/tpc.112.105767
通讯作者	Dai, Mingqiu
英文摘要	The basic Leucine zipper transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5) is a key regulator of abscisic acid (ABA)-mediated seed germination and postgermination seedling growth. While a family of SUCROSE NONFERMENTING1-related protein kinase2s (SnRK2s) is responsible for ABA-induced phosphorylation and stabilization of ABI5, the phosphatase(s) responsible for dephosphorylating ABI5 is still unknown. Here, we demonstrate that mutations in FyPP1 (for Phytochrome-associated serine/threonine protein phosphatase1) and FyPP3, two homologous genes encoding the catalytic subunits of Ser/Thr PROTEIN PHOSPHATASE6 (PP6), cause an ABA hypersensitive phenotype in Arabidopsis thaliana, including ABA-mediated inhibition of seed germination and seedling growth. Conversely, overexpression of FyPP causes reduced sensitivity to ABA. The ABA hypersensitive phenotype of FyPP loss-of-function mutants is ABI5 dependent, and the amount of phosphorylated and total ABI5 proteins inversely correlates with the levels of FyPP proteins. Moreover, FyPP proteins physically interact with ABI5 in vitro and in vivo, and the strength of the interaction depends on the ABI5 phosphorylation status. In vitro phosphorylation assays show that FyPP proteins directly dephosphorylate ABI5. Furthermore, genetic and biochemical assays show that FyPP proteins act antagonistically with SnRK2 kinases to regulate ABI5 phosphorylation and ABA responses. Thus, Arabidopsis PP6 phosphatase regulates ABA signaling through dephosphorylation and destabilization of ABI5.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; CELL BIOLOGY ; PLANT SCIENCES
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000316805700015
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/5071]
专题	作物科学研究所
作者单位	1.Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA 2.Wilkes Univ, Dept Biol, Wilkes Barre, PA 18766 USA 3.Pusan Natl Univ, Dept Biol Educ, Pusan 609735, South Korea 4.NE Normal Univ, State Environm Protect Key Lab Wetland Ecol & Veg, Changchun 130062, Peoples R China 5.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 6.Natl Engn Res Ctr Crop Mol Design, Beijing 100085, Peoples R China 7.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China
推荐引用方式 GB/T 7714	Dai, Mingqiu,Xue, Qin,Mccray, Tyra,et al. The PP6 Phosphatase Regulates ABI5 Phosphorylation and Abscisic Acid Signaling in Arabidopsis[J]. PLANT CELL,2013,25(2):517-534.
APA	Dai, Mingqiu.,Xue, Qin.,Mccray, Tyra.,Margavage, Kathryn.,Chen, Fang.,...&Wang, Haiyang.(2013).The PP6 Phosphatase Regulates ABI5 Phosphorylation and Abscisic Acid Signaling in Arabidopsis.PLANT CELL,25(2),517-534.
MLA	Dai, Mingqiu,et al."The PP6 Phosphatase Regulates ABI5 Phosphorylation and Abscisic Acid Signaling in Arabidopsis".PLANT CELL 25.2(2013):517-534.