Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity

doi:10.1016/S2095-3119(14)60785-1

CORC > 中国农业科学院 > 作物科学研究所

	Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity
	Yang Xiu-shi 1,2; Wang Li-jun 2,3; Dung Chuan 1; Lui, Edmund Man King 4; Ren Gui-xing 2
刊名	JOURNAL OF INTEGRATIVE AGRICULTURE
	2014
卷号	13 期号:12 页码:2807-2815
关键词	ultrasonic-assisted extraction polysaccharides American ginseng response surface methodology immunostimulating activity
ISSN号	2095-3119
DOI	10.1016/S2095-3119(14)60785-1
通讯作者	Yang Xiu-shi
英文摘要	Ultrasonic-assisted extraction (UAE) of American ginseng polysacchatides (AGP) was investigated using response surface methodology. Three-factor-three-level Box-Behnken design was employed to optimize the ultrasonic power, extraction time and ratio of water to raw material to obtain a high AGP yield. The analysis of variance and response surface plots indicated that ultrasonic power was the most important factor affecting the extraction yield. The optimal conditions were ultrasonic power 400 W, extraction time 71 min, and ratio of water to raw material 33 mL g(-1). Under these conditions, the yield of AGP was 8.09%, which was agreed closely to the predicted value. Gas chromatography (GC) analysis showed that AGP was composed of arabinose, rhamnose, galactose, glucose, and galacturonic acid. Fourier transform infrared spectra revealed the general characteristic absorption peaks of AGP. In addition, AGP exhibited good immunostimulating activities by up-regulating the production of nitric oxide and cytokines. Compared with hot water extraction, UAE required shorter extraction time and gave a higher extraction yield, without changing the structure and immunostimulating activity of AGP. The results indicated that UAE could be an effective and advisable technique for the large scale production of plant polysaccharides.
学科主题	Agriculture, Multidisciplinary
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000346628400024
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4964]
专题	作物科学研究所
作者单位	1.Shanxi Univ, Inst Environm Sci, Taiyuan 030006, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 3.Chinese Univ Hong Kong, Sch Life Sci, Food & Nutr Sci Programme, Shatin 999077, Hong Kong, Peoples R China 4.Univ Western Ontario, Schulich Sch Med & Dent, Dept Physiol & Pharmacol, London, ON N6A 5C1, Canada
推荐引用方式 GB/T 7714	Yang Xiu-shi,Wang Li-jun,Dung Chuan,et al. Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2014,13(12):2807-2815.
APA	Yang Xiu-shi,Wang Li-jun,Dung Chuan,Lui, Edmund Man King,&Ren Gui-xing.(2014).Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity.JOURNAL OF INTEGRATIVE AGRICULTURE,13(12),2807-2815.
MLA	Yang Xiu-shi,et al."Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity".JOURNAL OF INTEGRATIVE AGRICULTURE 13.12(2014):2807-2815.