OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity

doi:10.1007/s11248-014-9803-2

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	OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity
	Huang, Jiexue 1; Cai, Maohong 1; Long, Qizhang 1; Liu, Linglong 1; Lin, Qiuyun 1; Jiang, Ling 1; Chen, Saihua 1; Wan, Jianmin 1,2
刊名	TRANSGENIC RESEARCH
	2014
卷号	23 期号:4 页码:643-655
关键词	Oryza sativa L. OsLOX2 Seed germination Seed longevity Grain storage
ISSN号	0962-8819
DOI	10.1007/s11248-014-9803-2
通讯作者	Huang, Jiexue
英文摘要	Rice production and seed storage are confronted with grain deterioration and loss of seed viability. Some members of the lipoxygenase (LOX) family function in degradation of storage lipids during the seed germination, but little is known about their influence on seed longevity during storage. We characterized the role of rice OsLOX2 gene in seed germination and longevity via over-expression and knock-down approaches. Abundant expression of OsLOX2 was detected in panicles, roots, and stems, but not in leaves. Moreover, OsLOX2 was highly induced during germination. OsLOX2 protein, located in the cytoplasm, showed a wide range of temperature adaptation (20-50 A degrees C) and a substrate preference to linoleic acid. Lines over-expressing OsLOX2 showed accelerated seed germination under normal condition and lower seed viability after accelerated aging. RNA interference (RNAi) of OsLOX2 caused delayed germination and enhanced seed longevity. RNAi lines with strongly repressed OsLOX2 activity completely lost the capability of germination after accelerated aging. More lipid hydroperoxide were found in OE15 than the control, but less in RNAi lines than in the WT Nipponbare. Therefore, OsLOX2 acts in opposite directions during seed germination and longevity during storage. Appropriate repression of the OsLOX2 gene may delay the aging process during the storage without compromising germination under normal conditions.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; BIOCHEMICAL RESEARCH METHODS ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000337094700008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4852]
专题	作物科学研究所
作者单位	1.Nanjing Agr Univ, Jiangsu Prov Ctr Plant Gene Engn, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 10081, Peoples R China
推荐引用方式 GB/T 7714	Huang, Jiexue,Cai, Maohong,Long, Qizhang,et al. OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity[J]. TRANSGENIC RESEARCH,2014,23(4):643-655.
APA	Huang, Jiexue.,Cai, Maohong.,Long, Qizhang.,Liu, Linglong.,Lin, Qiuyun.,...&Wan, Jianmin.(2014).OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity.TRANSGENIC RESEARCH,23(4),643-655.
MLA	Huang, Jiexue,et al."OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity".TRANSGENIC RESEARCH 23.4(2014):643-655.