The last half-repeat of transcription activator-like effector (TALE) is dispensable and thereby TALE-based technology can be simplified

doi:10.1111/mpp.12125

CORC > 中国农业科学院 > 作物科学研究所

	The last half-repeat of transcription activator-like effector (TALE) is dispensable and thereby TALE-based technology can be simplified
	Zheng, Chong-Ke; Wang, Chun-Lian; Zhang, Xiao-Ping; Wang, Fu-Jun; Qin, Teng-Fei; Zhao, Kai-Jun
刊名	MOLECULAR PLANT PATHOLOGY
	2014
卷号	15 期号:7 页码:690-697
关键词	dTALE last half-repeat TAL effector TALENs unit assembly
ISSN号	1464-6722
DOI	10.1111/mpp.12125
通讯作者	Zheng, Chong-Ke
英文摘要	To activate the expression of host genes that contribute to pathogen growth, pathogenic Xanthomonas bacteria inject their transcription activator-like effectors (TALEs) into plant cells and the TALEs bind to target gene promoters by the central repeat region consisting of near-perfect 34-amino-acid repeats (34-aa repeats). Based on the recognition codes between the 34-aa repeats and the targeted nucleotides, TALE-based technologies, such as designer TALEs (dTALEs) and TALE nucleases (TALENs), have been developed. Amazingly, every natural TALE invariantly has a truncated last half-repeat (LHR) at the end of the 34-aa repeats. Consequently, all the reported dTALEs and TALENs also harbour their LHRs. Here, we show that the LHRs in dTALEs are dispensable for the function of gene activation by both transient expression assays in Nicotiana benthamiana and gene-specific targeting in the rice genome, indicating that TALEs might originate from a single progenitor. In the light of this finding, we demonstrate that dTALEs can be constructed through two simple steps. Moreover, the activation strengths of dTALEs lacking the LHR are comparable with those of dTALEs harbouring the LHR. Our results provide new insights into the origin of natural TALEs, and will facilitate the simplification of the design and assembly of TALE-based tools, such as dTALEs and TALENs, in the near future.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000340677400005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4843]
专题	作物科学研究所
作者单位	Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Key Lab Crop Genet & Breeding,Minist Agr, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Chong-Ke,Wang, Chun-Lian,Zhang, Xiao-Ping,et al. The last half-repeat of transcription activator-like effector (TALE) is dispensable and thereby TALE-based technology can be simplified[J]. MOLECULAR PLANT PATHOLOGY,2014,15(7):690-697.
APA	Zheng, Chong-Ke,Wang, Chun-Lian,Zhang, Xiao-Ping,Wang, Fu-Jun,Qin, Teng-Fei,&Zhao, Kai-Jun.(2014).The last half-repeat of transcription activator-like effector (TALE) is dispensable and thereby TALE-based technology can be simplified.MOLECULAR PLANT PATHOLOGY,15(7),690-697.
MLA	Zheng, Chong-Ke,et al."The last half-repeat of transcription activator-like effector (TALE) is dispensable and thereby TALE-based technology can be simplified".MOLECULAR PLANT PATHOLOGY 15.7(2014):690-697.