Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection

doi:10.1073/pnas.1421475112

CORC > 中国农业科学院 > 作物科学研究所

	Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection
	Qiao, Yongli 1,2,3; Shi, Jinxia 1,2; Zhai, Yi 1; Hou, Yingnan 1,2; Ma, Wenbo 1,2
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2015
卷号	112 期号:18 页码:5850-5855
关键词	Phytophthora pathogenesis RxLR effector RNA helicase gene silencing small RNA
ISSN号	0027-8424
DOI	10.1073/pnas.1421475112
通讯作者	Qiao, Yongli
英文摘要	A broad range of parasites rely on the functions of effector proteins to subvert host immune response and facilitate disease development. The notorious Phytophthora pathogens evolved effectors with RNA silencing suppression activity to promote infection in plant hosts. Here we report that the Phytophthora Suppressor of RNA Silencing 1 (PSR1) can bind to an evolutionarily conserved nuclear protein containing the aspartate-glutamate-alanine-histidine-box RNA helicase domain in plants. This protein, designated PSR1-Interacting Protein 1 (PINP1), regulates the accumulation of both microRNAs and endogenous small interfering RNAs in Arabidopsis. A null mutation of PINP1 causes embryonic lethality, and silencing of PINP1 leads to developmental defects and hypersusceptibility to Phytophthora infection. These phenotypes are reminiscent of transgenic plants expressing PSR1, supporting PINP1 as a direct virulence target of PSR1. We further demonstrate that the localization of the Dicer-like 1 protein complex is impaired in the nucleus of PINP1-silenced or PSR1-expressing cells, indicating that PINP1 may facilitate small RNA processing by affecting the assembly of dicing complexes. A similar function of PINP1 homologous genes in development and immunity was also observed in Nicotiana benthamiana. These findings highlight PINP1 as a previously unidentified component of RNA silencing that regulates distinct classes of small RNAs in plants. Importantly, Phytophthora has evolved effectors to target PINP1 in order to promote infection.
学科主题	Multidisciplinary Sciences
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000353953800079
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4714]
专题	作物科学研究所
作者单位	1.Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA 2.Univ Calif Riverside, Ctr Plant Cell Biol, Riverside, CA 92521 USA 3.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Yongli,Shi, Jinxia,Zhai, Yi,et al. Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2015,112(18):5850-5855.
APA	Qiao, Yongli,Shi, Jinxia,Zhai, Yi,Hou, Yingnan,&Ma, Wenbo.(2015).Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,112(18),5850-5855.
MLA	Qiao, Yongli,et al."Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112.18(2015):5850-5855.