The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH | |
Hao, Meng-Shu1; Jensen, Anna M.1,2; Boquist, Ann-Sofie1; Liu, Yun-Jun1,3; Rasmusson, Allan G.1 | |
刊名 | PLOS ONE |
2015 | |
卷号 | 10期号:9页码:- |
ISSN号 | 1932-6203 |
DOI | 10.1371/journal.pone.0139224 |
通讯作者 | Hao, Meng-Shu |
英文摘要 | NADPH is a key reductant carrier that maintains internal redox and antioxidant status, and that links biosynthetic, catabolic and signalling pathways. Plants have a mitochondrial external NADPH oxidation pathway, which depends on Ca2+ and pH in vitro, but concentrations of Ca2+ needed are not known. We have determined the K-0.5(Ca2+) of the external NADPH dehydrogenase from Solanum tuberosum mitochondria and membranes of E. coli expressing Arabidopsis thaliana NDB1 over the physiological pH range using O-2 and decylubiquinone as electron acceptors. The K-0.5(Ca2+) of NADPH oxidation was generally higher than for NADH oxidation, and unlike the latter, it depended on pH. At pH 7.5, K-0.5(Ca2+) for NADPH oxidation was high (approximate to 100 mu M), yet 20-fold lower K-0.5(Ca2+) values were determined at pH 6.8. Lower K-0.5(Ca2+) values were observed with decylubiquinone than with O-2 as terminal electron acceptor. NADPH oxidation responded to changes in Ca2+ concentrations more rapidly than NADH oxidation did. Thus, cytosolic acidification is an important activator of external NADPH oxidation, by decreasing the Ca2+-requirements for NDB1. The results are discussed in relation to the present knowledge on how whole cell NADPH redox homeostasis is affected in plants modified for the NDB1 gene. |
学科主题 | Multidisciplinary Sciences |
语种 | 英语 |
出版者 | PUBLIC LIBRARY SCIENCE |
WOS记录号 | WOS:000362170700050 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/4658] |
专题 | 作物科学研究所 |
作者单位 | 1.Lund Univ, Dept Biol, Lund, Sweden 2.Linnaeus Univ, Dept Forestry & Wood Technol, Vaxjo, Sweden 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100193, Peoples R China |
推荐引用方式 GB/T 7714 | Hao, Meng-Shu,Jensen, Anna M.,Boquist, Ann-Sofie,et al. The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH[J]. PLOS ONE,2015,10(9):-. |
APA | Hao, Meng-Shu,Jensen, Anna M.,Boquist, Ann-Sofie,Liu, Yun-Jun,&Rasmusson, Allan G..(2015).The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH.PLOS ONE,10(9),-. |
MLA | Hao, Meng-Shu,et al."The Ca2+-Regulation of the Mitochondrial External NADPH Dehydrogenase in Plants Is Controlled by Cytosolic pH".PLOS ONE 10.9(2015):-. |
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