A rice White-stripe leaf3 (wsl3) mutant lacking an HD domain-containing protein affects chlorophyll biosynthesis and chloroplast development

doi:10.1007/s12374-016-0459-8

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	A rice White-stripe leaf3 (wsl3) mutant lacking an HD domain-containing protein affects chlorophyll biosynthesis and chloroplast development
	Zhao, Shaolu 1; Long, Wuhua 1; Wang, Yihua 1; Liu, Linglong 1; Wang, Yunlong 1; Niu, Mei 1; Zheng, Ming 1; Wang, Di 1; Wan, Jianmin 1,2
刊名	JOURNAL OF PLANT BIOLOGY
	2016
卷号	59 期号:3 页码:282-292
关键词	Chlorophyll Chloroplast HD domain-containing protein Rice (Oryza sativa) White-Stripe Leaf 3
ISSN号	1226-9239
DOI	10.1007/s12374-016-0459-8
通讯作者	Zhao, Shaolu
英文摘要	Leaf-color mutants are ideal genetic materials for understanding the mechanism of chloroplast development and chlorophyll (Chl) biosynthesis. Here we isolated and identified a new leaf-color mutant of rice, named white-stripe leaf3 (wsl3), from a Co-60-irradiated mutant pool. The wsl3 mutant displayed a visible white-stripe leaf in both young seedlings and flag leaves of mature plant. Chl content in homozygous wsl3 mutant was approximately 47% of that in the wild type. Besides, chloroplast development in the mutant was severely arrested. By a map-based cloning strategy, the wsl3 gene was finely confined to a 50.8 kb region on chromosome 1. Moreover, a 9-bp deletion was identified in the genomic region of LOC_Os01g01920, which encodes an HD (histidine and aspartic acid) domaincontaining protein. Genetic complementation confirmed that LOC_Os01g01920 could recover the lesion of wsl3 mutation. Real-time PCR analyses showed that the expression levels of WSL3 were the highest in young and flag leaves among various tissues, and most of the genes associated with Chl biosynthesis were significantly down-regulated in the wsl3 mutant. Meanwhile, in contrast to many nuclear gene-encoded phage-type RNA polymerase(s) (NEP) transcribed genes were up-regulated, most of plastid-encoded bacterialtype RNA polymerase (PEP) transcribed genes were downregulated. These results demonstrated that the WSL3 gene, as an HD domain-containing protein, is involved in chl biosynthesis and chloroplast development in rice.
学科主题	Plant Sciences
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000377411800009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/4512]
专题	作物科学研究所
作者单位	1.Nanjing Agr Univ, Jiangsu Plant Gene Engn Res Ctr, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Shaolu,Long, Wuhua,Wang, Yihua,et al. A rice White-stripe leaf3 (wsl3) mutant lacking an HD domain-containing protein affects chlorophyll biosynthesis and chloroplast development[J]. JOURNAL OF PLANT BIOLOGY,2016,59(3):282-292.
APA	Zhao, Shaolu.,Long, Wuhua.,Wang, Yihua.,Liu, Linglong.,Wang, Yunlong.,...&Wan, Jianmin.(2016).A rice White-stripe leaf3 (wsl3) mutant lacking an HD domain-containing protein affects chlorophyll biosynthesis and chloroplast development.JOURNAL OF PLANT BIOLOGY,59(3),282-292.
MLA	Zhao, Shaolu,et al."A rice White-stripe leaf3 (wsl3) mutant lacking an HD domain-containing protein affects chlorophyll biosynthesis and chloroplast development".JOURNAL OF PLANT BIOLOGY 59.3(2016):282-292.