Surface plasmon resonance and electrochemistry characterization oflayer-by-layer self-assembled DNA and Zr4+ thin films,and their interaction with cytochrome c

	Surface plasmon resonance and electrochemistry characterization oflayer-by-layer self-assembled DNA and Zr4+ thin films,and their interaction with cytochrome c
	Wang HL ; Wang F ; Xu Z ; Wang YZ ; Dong SJ
刊名	talanta
	2007
卷号	74 期号:1 页码:104-109
关键词	MULTILAYER FILMS GOLD NANOPARTICLES SUCCESSIVE DEPOSITION MODIFIED ELECTRODE ALTERNATE LAYERS ULTRATHIN FILMS GLUCOSE-OXIDASE HYBRID FILMS ADSORPTION FABRICATION
ISSN号	0039-9140
通讯作者	dong sj
中文摘要	through electrostatic layer-by-layer (lbl) assembly, negatively charged calf thymus double stranded dna (ctds-dna), and positively charged zr4+ ions were alternately deposited on gold substrate modified with chemisorbed cysteamine. thus-prepared three-dimensional dna networks were characterized by surface plasmon resonance (spr) spectroscopy, x-ray photoelectron spectroscopy (xps) and infrared reflection-absorption spectroscopy (ir-ras). spr spectroscopy indicates that the effective thickness of dna monolayer in the (dna/zr4+), bilayer was 1.5 +/- 0.1 nm, which corresponds to the surface coverage of 79% of its full packed monolayer. at the same time, a linear increase of film thickness with increasing number of layers was also confirmed by spr characterizations. the data of xps and ir-ras show that zr4+ ions interact with both the phosphate groups and nitrogenous bases of dna and load into the framework of dna. furthermore, the interactions between this composite film and heme protein cytochrome c (cyt c) were investigated by spr spectroscopy and electrochemistry. compared with the adsorption of cyt c on dna monolayer, this composite multilayer film can obviously enhance the amount of immobilized cyt c confirmed by spr reflectivity-incident angle (r-theta) curves.
收录类别	其它国内期刊或国际会议论文
语种	英语
公开日期	2010-07-13 ; 2011-06-09
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/13585]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang HL,Wang F,Xu Z,et al. Surface plasmon resonance and electrochemistry characterization oflayer-by-layer self-assembled DNA and Zr4+ thin films,and their interaction with cytochrome c[J]. talanta,2007,74(1):104-109.
APA	Wang HL,Wang F,Xu Z,Wang YZ,&Dong SJ.(2007).Surface plasmon resonance and electrochemistry characterization oflayer-by-layer self-assembled DNA and Zr4+ thin films,and their interaction with cytochrome c.talanta,74(1),104-109.
MLA	Wang HL,et al."Surface plasmon resonance and electrochemistry characterization oflayer-by-layer self-assembled DNA and Zr4+ thin films,and their interaction with cytochrome c".talanta 74.1(2007):104-109.