Enhanced immune response to inactivated porcine circovirus type 2 (PCV2) vaccine by conjugation of chitosan oligosaccharides | |
Zhang, Guiqiang1,2,3; Jia, Peiyuan1,2; Cheng, Gong1,2; Jiao, Siming1,2; Ren, Lishi1,2; Ji, Shaoyang1,2; Hu, Tao1,2; Liu, Hongtao1,2; Du, Yuguang1,2 | |
刊名 | CARBOHYDRATE POLYMERS |
2017-06-15 | |
卷号 | 166页码:64-72 |
关键词 | Porcine Circovirus Type 2 (Pcv2) Vaccine Adjuvant Chitosan Oligosaccharide Conjugation |
ISSN号 | 0144-8617 |
DOI | 10.1016/j.carbpol.2017.02.058 |
文献子类 | Article |
英文摘要 | This study aimed to investigate the effect of chitosan oligosaccharide (COS) conjugation on the immunogenicity of porcine circovirus type-2 (PCV2) vaccine. Two conjugates (PCV2-COS-1 and PCV2-COS-2) were designed by covalent conjugation of an inactivated PCV2 vaccine with COS, and administered to C57BL/6 mice three times at two-week intervals. The results indicate that, as compared to PCV2 alone group, the PCV2-COS conjugates remarkably enhanced both humoral and cellular immunity against PCV2 by promoting T lymphocyte proliferation and initiating a mixed Th1/Th2 response, including the elevated production of PCV-2 specific antibodies and up-regulated secretion of inflammatory cytoldnes. Noticeably, the immunization with PCV2-COS-1 conjugate displayed similar or even better immune-stimulating effects than that by PCV2/ISA206 (a commercialized adjuvant) and showed no infection or pathological signs at injection sites of the mice. Presumably, the covalent linkage of PCV2 vaccine to COS might be a viable strategy to increase the efficacy against PCV2-associated diseases. (C) 2017 Elsevier Ltd. All rights reserved. |
WOS关键词 | T-cell Immunity ; Dendritic Cells ; Antigen ; Receptor ; Mice ; Immunogenicity ; Efficacy ; Viremia ; Ligand ; Pigs |
WOS研究方向 | Chemistry ; Polymer Science |
语种 | 英语 |
WOS记录号 | WOS:000400213300007 |
资助机构 | National Programs for High Technology Research and Development (863 Programs)(2014AA093604) ; National Natural Science Fund of China(31500747 ; Postdoctoral Science Fund of China(2014M561069) ; 31570801) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/22817] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, PLA, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, PLA, Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Guiqiang,Jia, Peiyuan,Cheng, Gong,et al. Enhanced immune response to inactivated porcine circovirus type 2 (PCV2) vaccine by conjugation of chitosan oligosaccharides[J]. CARBOHYDRATE POLYMERS,2017,166:64-72. |
APA | Zhang, Guiqiang.,Jia, Peiyuan.,Cheng, Gong.,Jiao, Siming.,Ren, Lishi.,...&Du, Yuguang.(2017).Enhanced immune response to inactivated porcine circovirus type 2 (PCV2) vaccine by conjugation of chitosan oligosaccharides.CARBOHYDRATE POLYMERS,166,64-72. |
MLA | Zhang, Guiqiang,et al."Enhanced immune response to inactivated porcine circovirus type 2 (PCV2) vaccine by conjugation of chitosan oligosaccharides".CARBOHYDRATE POLYMERS 166(2017):64-72. |
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