Knocking-Down Cyclin A(2) by siRNA Suppresses Apoptosis and Switches Differentiation Pathways in K562 Cells upon Administration with Doxorubicin

	Knocking-Down Cyclin A(2) by siRNA Suppresses Apoptosis and Switches Differentiation Pathways in K562 Cells upon Administration with Doxorubicin
	Wang XH ; Song YJ ; Ren JS ; Qu XG
刊名	plos one
	2009
卷号	4 期号:8 页码:文献编号：e6665
ISSN号	1932-6203
通讯作者	wang xh
中文摘要	cyclin a(2) is critical for the initiation of dna replication, transcription and cell cycle regulation. cumulative evidences indicate that the deregulation of cyclin a(2) is tightly linked to the chromosomal instability, neoplastic transformation and tumor proliferation. here we report that treatment of chronic myelogenous leukaemia k562 cells with doxorubicin results in an accumulation of cyclin a(2) and follows by induction of apoptotic cell death. to investigate the potential preclinical relevance, k562 cells were transiently transfected with the sirna targeting cyclin a(2) by functionalized single wall carbon nanotubes. knocking down the expression of cyclin a(2) in k562 cells suppressed doxorubicin-induced growth arrest and cell apoptosis. upon administration with doxorubicin, k562 cells with reduced cyclin a(2) showed a significant decrease in erythroid differentiation, and a small fraction of cells were differentiated along megakaryocytic and monocyte-macrophage pathways. the results demonstrate the pro-apoptotic role of cyclin a(2) and suggest that cyclin a(2) is a key regulator of cell differentiation.
收录类别	其它国际期刊论文
语种	英语
WOS记录号	WOS:000269075600002
公开日期	2010-06-18
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/12713]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang XH,Song YJ,Ren JS,et al. Knocking-Down Cyclin A(2) by siRNA Suppresses Apoptosis and Switches Differentiation Pathways in K562 Cells upon Administration with Doxorubicin[J]. plos one,2009,4(8):文献编号：e6665.
APA	Wang XH,Song YJ,Ren JS,&Qu XG.(2009).Knocking-Down Cyclin A(2) by siRNA Suppresses Apoptosis and Switches Differentiation Pathways in K562 Cells upon Administration with Doxorubicin.plos one,4(8),文献编号：e6665.
MLA	Wang XH,et al."Knocking-Down Cyclin A(2) by siRNA Suppresses Apoptosis and Switches Differentiation Pathways in K562 Cells upon Administration with Doxorubicin".plos one 4.8(2009):文献编号：e6665.