Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes

	Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes
	Li T ; Dong SJ ; Wang E
刊名	analytical chemistry
	2009
卷号	81 期号:6 页码:2144-2149
关键词	FUNCTIONALIZED GOLD NANOPARTICLES SELECTIVE DETECTION PEROXIDASE-ACTIVITY TELOMERASE ACTIVITY AMPLIFIED ANALYSIS CATALYTIC BEACONS OPTICAL-DETECTION METAL-IONS DNA COMPLEX
ISSN号	0003-2700
通讯作者	wang e
中文摘要	mercury ion (hg2+) is able to specifically bind to the thymine-thymine (t-t) base pair in a dna duplex, thus providing a rationale for dna-based selective detection of hg2+ with various means. in this work, we for the first time utilize the hg2+-mediated t-t base pair to modulate the proper folding of g-quadruplex dnas and inhibit the dnazyme activity, thereby pioneering a facile approach to sense hg2+ with colorimetry. two bimolecular dna g-quadruplexes containing many t residues are adopted here, which function well in low- and high-salt conditions, respectively. these g-quadruplex dnas are able to bind hemin to form the peroxidase-like dnazymes in the folded state. upon addition of hg2+, the proper folding of g-quadruplex dnas is inhibited due to the formation of t-hg2+-t complex. ibis is reflected by the notable change of the soret band of hemin when investigated by using uv-vis absorption spectroscopy. as a result of hg2+ inhibition, a sharp decrease in the catalytic activity toward the h2o2-mediated oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (abts) is observed, accompanied by a change in solution color. through this approach, aqueous hg2+ can be detected at 50 nm (10 ppb) with colorimetry in a facile way, with high selectivity against other metal ions.
收录类别	SCI
语种	英语
WOS记录号	WOS:000264142000015
公开日期	2010-06-18
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/12681]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li T,Dong SJ,Wang E. Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes[J]. analytical chemistry,2009,81(6):2144-2149.
APA	Li T,Dong SJ,&Wang E.(2009).Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes.analytical chemistry,81(6),2144-2149.
MLA	Li T,et al."Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes".analytical chemistry 81.6(2009):2144-2149.