Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes | |
Li T ; Dong SJ ; Wang E | |
刊名 | analytical chemistry
![]() |
2009 | |
卷号 | 81期号:6页码:2144-2149 |
关键词 | FUNCTIONALIZED GOLD NANOPARTICLES SELECTIVE DETECTION PEROXIDASE-ACTIVITY TELOMERASE ACTIVITY AMPLIFIED ANALYSIS CATALYTIC BEACONS OPTICAL-DETECTION METAL-IONS DNA COMPLEX |
ISSN号 | 0003-2700 |
通讯作者 | wang e |
中文摘要 | mercury ion (hg2+) is able to specifically bind to the thymine-thymine (t-t) base pair in a dna duplex, thus providing a rationale for dna-based selective detection of hg2+ with various means. in this work, we for the first time utilize the hg2+-mediated t-t base pair to modulate the proper folding of g-quadruplex dnas and inhibit the dnazyme activity, thereby pioneering a facile approach to sense hg2+ with colorimetry. two bimolecular dna g-quadruplexes containing many t residues are adopted here, which function well in low- and high-salt conditions, respectively. these g-quadruplex dnas are able to bind hemin to form the peroxidase-like dnazymes in the folded state. upon addition of hg2+, the proper folding of g-quadruplex dnas is inhibited due to the formation of t-hg2+-t complex. ibis is reflected by the notable change of the soret band of hemin when investigated by using uv-vis absorption spectroscopy. as a result of hg2+ inhibition, a sharp decrease in the catalytic activity toward the h2o2-mediated oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (abts) is observed, accompanied by a change in solution color. through this approach, aqueous hg2+ can be detected at 50 nm (10 ppb) with colorimetry in a facile way, with high selectivity against other metal ions. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000264142000015 |
公开日期 | 2010-06-18 |
内容类型 | 期刊论文 |
源URL | [http://202.98.16.49/handle/322003/12681] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Li T,Dong SJ,Wang E. Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes[J]. analytical chemistry,2009,81(6):2144-2149. |
APA | Li T,Dong SJ,&Wang E.(2009).Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes.analytical chemistry,81(6),2144-2149. |
MLA | Li T,et al."Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes".analytical chemistry 81.6(2009):2144-2149. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论