Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma

	Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma
	Shang L ; Yin JY ; Li J ; Jin LH ; Dong SJ
刊名	biosensors & bioelectronics
	2009
卷号	25 期号:2 页码:269-274
关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY RESONANCE ENERGY-TRANSFER SELECTIVE DETECTION CAPILLARY-ELECTROPHORESIS MASS-SPECTROMETRY IN-VIVO CYSTEINE HOMOCYSTEINE SENSOR PROBES
ISSN号	0956-5663
通讯作者	dong sj
中文摘要	in this work, a new fluorescent method for sensitive detection of biological thiols in human plasma was developed using a near-infrared (nir) fluorescent dye, fr 730. the sensing approach was based on the strong affinity of thiols to gold and highly efficient fluorescent quenching ability of gold nanoparticles (au nps). in the presence of thiols, the nir fluorescence would enhance dramatically due to desorption of fr 730 from the surfaces of au nps, which allowed the analysis of thiol-containing amino acids in a very simple approach. the size of au nps was found to affect the fluorescent assay and the best response for cysteine detection was achieved when using au nps with the diameter of 24 nm, where a linear range of 2.5 x 10(-8) m to 4.0 x 10(-6) m and a detection limit of as low as 10 nm was obtained. this method also demonstrated a high selectivity to thiol-containing amino acids due to the strong affinity of thiols to gold.
收录类别	SCI
语种	英语
公开日期	2010-05-27 ; 2011-06-09
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/11931]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Shang L,Yin JY,Li J,et al. Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma[J]. biosensors & bioelectronics,2009,25(2):269-274.
APA	Shang L,Yin JY,Li J,Jin LH,&Dong SJ.(2009).Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma.biosensors & bioelectronics,25(2),269-274.
MLA	Shang L,et al."Gold nanoparticle-based near-infrared fluorescent detection of biological thiols in human plasma".biosensors & bioelectronics 25.2(2009):269-274.