Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 单细胞中心

	Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose
	Zhang, Biao 1; Zhu, Yelin 1; Zhang, Jia 2; Wang, Dongmei 1; Sun, Lianhong 1; Hong, Jiong 1
刊名	BIORESOURCE TECHNOLOGY
	2017
卷号	224 页码:553-562
关键词	Kluyveromyces marxianus Pyruvate Xylose Elevated temperature Cofermentation
英文摘要	Xylose and glucose from lignocellulose are sustainable sources for production of pyruvate, which is the starting material for the synthesis of many drugs and agrochemicals. In this study, the pyruvate decarboxylase gene (KmPDC1) and glycerol-3-phosphate dehydrogenase gene (KmGPD1) of Kluyveromyces marxianus YZJ051 were disrupted to prevent ethanol and glycerol accumulation. The deficient growth of PDC disruption was rescued by overexpressing mutant KmMTH1-Delta T. Then pentose phosphate pathway and xylitol dehydrogenase SsXYL2-ARS genes were overexpressed to obtain strain YZB053 which produced pyruvate with xylose other than glucose. It produced 24.62 g/L pyruvate from 80 g/L xylose with productivity of 0.51 g/L/ h at 42 degrees C. Then, xylose-specific transporter ScGAL2-N376F was overexpressed to obtain strain YZB058, which simultaneously consumed 40g/L glucose and 20 g/L xylose and produced 29.21 g/L pyruvate with productivity of 0.81 g/L/h at 42 degrees C. Therefore, a platform for pyruvate production from glucose and xylose at elevated temperature was developed. (C) 2016 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; ETHANOL-PRODUCTION ; ESCHERICHIA-COLI ; SIMULTANEOUS SACCHARIFICATION ; XYLITOL PRODUCTION ; YEAST-STRAIN ; FERMENTATION ; PATHWAY ; THERMOTOLERANT ; OVEREXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000395691900067
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9338]
专题	青岛生物能源与过程研究所_单细胞中心
作者单位	1.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Biao,Zhu, Yelin,Zhang, Jia,et al. Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose[J]. BIORESOURCE TECHNOLOGY,2017,224:553-562.
APA	Zhang, Biao,Zhu, Yelin,Zhang, Jia,Wang, Dongmei,Sun, Lianhong,&Hong, Jiong.(2017).Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose.BIORESOURCE TECHNOLOGY,224,553-562.
MLA	Zhang, Biao,et al."Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose".BIORESOURCE TECHNOLOGY 224(2017):553-562.