Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum
	Yang, Dinglong 1,3; Wang, Qing1 ; Chen, Lizhu 1,3; Liu, Yongliang 1,2; Cao, Ruiwen 1,3; Wu, Huifeng1 ; Li, Fei1 ; Ji, Chenglong1 ; Cong, Ming1 ; Zhao, Jianmin1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2017-06-01
卷号	65 页码:17-24
关键词	Ruditapes philippinarum Phage-type lysozyme Tissue distribution Antimicrobial activity
ISSN号	1050-4648
英文摘要	A phage-type lysozyme, designed as RpPLYZ, was cloned and characterized from the clam Ruditapes philippinarum. The full-length cDNA of RpPLYZ was of 699 bp with an open reading frame (ORF) of 534 bp, encoding a polypeptide of 177-amino acid with a calculated molecular mass of 19.6 kDa and an isoelectric point of 9.05. Multiple alignments and phylogenetic analysis strongly suggested that RpPLYZ was a new member of the phage-type lysozyme family. The mRNA transcript of RpPLYZ was found to be constitutively expressed in a wide range of tissues and mainly in hemocytes and mantle. The relative expression of RpPLYZ mRNA in hemocytes was significantly up-regulated at 6, 24, 48 and 72 h after Vibrio anguillarum challenge. The recombinant RpPLYZ (rRpPLYZ) showed high activity against Entherobacter cloacae and Staphyloccocus aureus, and less effective towards Entherobacter aerogenes and V. anguillarum. Moreover, the optimal pH, temperature and ionic strength for rRpPLYZ activity was determined to be 5.5, 50 degrees C and 5 mM, respectively. These results suggested that RpPLYZ was a member of the phage-type lysozyme family and perhaps played an important role in the immune responses against bacterial invasion. (C) 2017 Elsevier Ltd. All rights reserved.
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	INVERTEBRATE-TYPE LYSOZYME ; I-TYPE LYSOZYMES ; ANTIMICROBIAL ACTIVITY ; CRASSOSTREA-VIRGINICA ; EXPRESSION ANALYSIS ; PENAEUS-MONODON ; EASTERN OYSTER ; CDNA CLONING ; DISK ABALONE ; TIGER SHRIMP
收录类别	SCI
语种	英语
WOS记录号	WOS:000402943200003
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/22526]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室海岸带生物资源高效利用研究与发展中心
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yang, Dinglong,Wang, Qing,Chen, Lizhu,et al. Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2017,65:17-24.
APA	Yang, Dinglong.,Wang, Qing.,Chen, Lizhu.,Liu, Yongliang.,Cao, Ruiwen.,...&Zhao, Jianmin.(2017).Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,65,17-24.
MLA	Yang, Dinglong,et al."Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 65(2017):17-24.