Effects of chronic ammonia exposure on ammonia metabolism and excretion in marine medaka Oryzias melastigma

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Effects of chronic ammonia exposure on ammonia metabolism and excretion in marine medaka Oryzias melastigma
	Gao, N; Zhu, LM; Guo, ZQ; Yi, MS; Zhang, L
刊名	FISH & SHELLFISH IMMUNOLOGY
	2017
卷号	65 页码:226-234
关键词	Oryzias melastigma Chronic exposure Toxicity Ammonia excretion Amino acid catabolism
通讯作者	yimsh@mail.sysu.edu.cn ; zhangli@scsio.ac.cn
中文摘要	Ammonia is highly toxic to aquatic organisms, but whether ammonia excretion or ammonia metabolism to less toxic compounds is the major strategy for detoxification in marine fish against chronic ammonia exposure is unclear to date. In this study, we investigated the metabolism and excretion of ammonia in marine medaka Oryzias melastigma during chronic ammonia exposure. The fish were exposed to 0, 0.1, 0.3, 0.6, and 1.1 mmol l(-1) NH4Cl spiked seawater for 8 weeks. Exposure of 0.3-1.1 mmol l(-1) NH4Cl had deleterious effects on the fish, including significant reductions in growth, feed intake, and total protein content. However, the fish could take strategies to detoxify ammonia. The tissue ammonia (T-Amm) in the 0.3-1.1 mmol l(-1) NH4Cl treatments was significantly higher than those in the 0 and 0.1 mmol l(-1) NH4Cl treatments after 2 weeks of exposure, but it recovered with prolonged exposure time, ultimately reaching the control level after 8 weeks. The amino acid catabolic rate decreased to reduce the gross ammonia production with the increasing ambient ammonia concentration. The concentrations of most metabolites remained constant in the 0-0.6 mmol l(-1) NH4Cl treatments, whereas 5 amino acids and 3 energy metabolism-related metabolites decreased in the 1.1 mmol l(-1) NH4Cl treatment. J(Amm) steadily increased in ambient ammonia from 0 to 0.6 mmol l(-1) and slightly decreased when the ambient ammonia concentration increased to 1.1 mmol l(-1). Overall, marine medaka cope with sublethal ammonia environment by regulating the tissue T-Amm via reducing the ammonia production and increasing ammonia excretion. (C) 2017 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/16291]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Gao, N,Zhu, LM,Guo, ZQ,et al. Effects of chronic ammonia exposure on ammonia metabolism and excretion in marine medaka Oryzias melastigma[J]. FISH & SHELLFISH IMMUNOLOGY,2017,65:226-234.
APA	Gao, N,Zhu, LM,Guo, ZQ,Yi, MS,&Zhang, L.(2017).Effects of chronic ammonia exposure on ammonia metabolism and excretion in marine medaka Oryzias melastigma.FISH & SHELLFISH IMMUNOLOGY,65,226-234.
MLA	Gao, N,et al."Effects of chronic ammonia exposure on ammonia metabolism and excretion in marine medaka Oryzias melastigma".FISH & SHELLFISH IMMUNOLOGY 65(2017):226-234.