Molecular characterization of Pacific white shrimp (Litopenaeus vannamei) sodium bicarbonate cotransporter (NBC) and its role in response to pH stress

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	Molecular characterization of Pacific white shrimp (Litopenaeus vannamei) sodium bicarbonate cotransporter (NBC) and its role in response to pH stress
	Cai, YM; Chen, T; Ren, CH; Huang, W; Jiang, X; Gao, Y; Huo, D; Hu, CQ
刊名	FISH & SHELLFISH IMMUNOLOGY
	2017
卷号	64 页码:226-233
关键词	Sodium bicarbonate cotransporter Litopenaeus vannamei In situ hybridization pH stress RNA interference Acid/base homeostasis
通讯作者	cym052065@163.com ; chan1010@scsio.ac.cn ; rosemary166@sina.com ; huangwen@scsio.ac.cn ; jiangxiao@scsio.ac.cn ; gaoyan13@mails.ucas.ac.cn ; huoda215@mails.ucas.ac.cn ; hucq@scsio.ac.cn
中文摘要	The sodium bicarbonate cotransporter (NBC) is an integral membrane ion transporter that can transport HCO3- (or a related species, such as CO32- across the plasma membrane. Previous researches revealed that NBC might play an important role in the regulation of intracellular pH in vertebrates. In the present study, an NBC cDNA was identified from Pacific white shrimp (Litopenaeus vannamei) and designated as Lv-NBC The full-length Lv-NBC cDNA is 4479 bp in size, containing a 5 '-untranslated region (UTR) of 59 bp, a 3 '-UTR of 835 bp and an open reading frame (ORF) of 3585 bp that encodes a protein of 1194 amino acids with a deduced molecular weight of 134.34 kDa. The Lv-NBC protein contains two functional domains (Band_3_cyto and HCO3_cotransp) and twelve transmembrane (TM) domains. Expression of the Lv-NBC mRNA was ubiquitously detected in all selected tissues, with the highest level in the gill. By in situ hybridization (ISH) with Digoxigenin-labeled probe, the Lv-NBC positive cells were shown mainly located in the secondary gill filaments. After low or high pH challenge, the transcript levels of Lv-NBC in the gill were found to be up-regulated. After knockdown of the Lv-NBC level by siRNA, the mortality of shrimp significantly increased under pH stress. Our study, as a whole, may provide evidences for the role of NBC in shrimp responding to pH stress, and give a new insight of the acid/base homeostasis mechanism in crustaceans. (C) 2017 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/16274]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Cai, YM,Chen, T,Ren, CH,et al. Molecular characterization of Pacific white shrimp (Litopenaeus vannamei) sodium bicarbonate cotransporter (NBC) and its role in response to pH stress[J]. FISH & SHELLFISH IMMUNOLOGY,2017,64:226-233.
APA	Cai, YM.,Chen, T.,Ren, CH.,Huang, W.,Jiang, X.,...&Hu, CQ.(2017).Molecular characterization of Pacific white shrimp (Litopenaeus vannamei) sodium bicarbonate cotransporter (NBC) and its role in response to pH stress.FISH & SHELLFISH IMMUNOLOGY,64,226-233.
MLA	Cai, YM,et al."Molecular characterization of Pacific white shrimp (Litopenaeus vannamei) sodium bicarbonate cotransporter (NBC) and its role in response to pH stress".FISH & SHELLFISH IMMUNOLOGY 64(2017):226-233.