Microarray-Based Study of Carbohydrate-Protein Binding by Gold Nanoparticle Probes | |
Gao JQ ; Liu DJ ; Wang ZX | |
刊名 | analytical chemistry |
2008 | |
卷号 | 80期号:22页码:8822–8827 |
关键词 | SURFACE-PLASMON RESONANCE E-SELECTIN CELL-ADHESION DNA CHIPS RECOGNITION LIGANDS LECTIN ANTIGEN OLIGOSACCHARIDES AFFINITY |
ISSN号 | 0003-2700 |
通讯作者 | wang zx |
中文摘要 | in order to develop a novel high-throughput tool for monitoring carbohydrate-protein interactions, we prepared carbohydrate or glycoprotein microarrays by immobilizing amino modified carbohydrates on aldehyde-derivatized glass slides or glycoprotein on epoxide-derivatized glass slides and carried out lectin binding experiments by using these microarrays, respectively. the interaction events are marked by attachment of gold nanoparticles followed by silver deposition for signal enhancement. the attachment of the gold nanoparticles is achieved by standard avidin-biotin chemistry. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000260910900061 |
公开日期 | 2010-04-14 |
内容类型 | 期刊论文 |
源URL | [http://ir.ciac.jl.cn/handle/322003/10681] |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Gao JQ,Liu DJ,Wang ZX. Microarray-Based Study of Carbohydrate-Protein Binding by Gold Nanoparticle Probes[J]. analytical chemistry,2008,80(22):8822–8827. |
APA | Gao JQ,Liu DJ,&Wang ZX.(2008).Microarray-Based Study of Carbohydrate-Protein Binding by Gold Nanoparticle Probes.analytical chemistry,80(22),8822–8827. |
MLA | Gao JQ,et al."Microarray-Based Study of Carbohydrate-Protein Binding by Gold Nanoparticle Probes".analytical chemistry 80.22(2008):8822–8827. |
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