Microarray-Based Study of Carbohydrate-Protein Binding by Gold Nanoparticle Probes
Gao JQ ; Liu DJ ; Wang ZX
刊名analytical chemistry
2008
卷号80期号:22页码:8822–8827
关键词SURFACE-PLASMON RESONANCE E-SELECTIN CELL-ADHESION DNA CHIPS RECOGNITION LIGANDS LECTIN ANTIGEN OLIGOSACCHARIDES AFFINITY
ISSN号0003-2700
通讯作者wang zx
中文摘要in order to develop a novel high-throughput tool for monitoring carbohydrate-protein interactions, we prepared carbohydrate or glycoprotein microarrays by immobilizing amino modified carbohydrates on aldehyde-derivatized glass slides or glycoprotein on epoxide-derivatized glass slides and carried out lectin binding experiments by using these microarrays, respectively. the interaction events are marked by attachment of gold nanoparticles followed by silver deposition for signal enhancement. the attachment of the gold nanoparticles is achieved by standard avidin-biotin chemistry.
收录类别SCI
语种英语
WOS记录号WOS:000260910900061
公开日期2010-04-14
内容类型期刊论文
源URL[http://ir.ciac.jl.cn/handle/322003/10681]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式
GB/T 7714
Gao JQ,Liu DJ,Wang ZX. Microarray-Based Study of Carbohydrate-Protein Binding by Gold Nanoparticle Probes[J]. analytical chemistry,2008,80(22):8822–8827.
APA Gao JQ,Liu DJ,&Wang ZX.(2008).Microarray-Based Study of Carbohydrate-Protein Binding by Gold Nanoparticle Probes.analytical chemistry,80(22),8822–8827.
MLA Gao JQ,et al."Microarray-Based Study of Carbohydrate-Protein Binding by Gold Nanoparticle Probes".analytical chemistry 80.22(2008):8822–8827.
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