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	Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins
	Wang YB(王粤博) ; Guo YL(郭寅龙) ; Xia XH(夏兴华)
刊名	J. Am. Soc. Mass Spectrom.
	2005
卷号	16期号:9页码:1488-1492
ISSN号	1044-0305
其他题名	多孔氧化铝膜作为样品载体用于基质辅助激光解吸附飞行时间质谱分析含盐蛋白质
通讯作者	王粤博
英文摘要	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF MS) analysis of proteins in salt-containing solution was performed for the first time using porous anodic alumina (PAA) membrane as sample support. The resulting spectral quality of proteins under standard sample preparation conditions was superior to that of normal metal sample stages. Analysis of phosphate-doped protein solutions indicated that porous anodic alumina membranes as a target yielded better results than a metallic target for salt-containing solutions. Because of the biocompatibility of the PAA, proteins can be adsorbed on the PAA and thus a washing process can be introduced to remove the salts from the PAA target before MS analysis. This desalting step significantly enhanced spectral quality, and better signal-to-noise ratios were obtained. The present technique is promising for proteomics research.
学科主题	分析化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.jasms.2005.04.014
语种	英语
WOS记录号	WOS:000231646500010
公开日期	2013-02-25
内容类型	期刊论文
源URL	[http://202.127.28.38/handle/331003/19884]
专题	上海有机化学研究所_分析化学研究室
推荐引用方式 GB/T 7714	Wang YB,Guo YL,Xia XH. Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins[J]. J. Am. Soc. Mass Spectrom.,2005,16(9):1488-1492.
APA	王粤博,郭寅龙,&夏兴华.(2005).Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins.J. Am. Soc. Mass Spectrom.,16(9),1488-1492.
MLA	王粤博,et al."Porous anodic alumina membrane as a sample support for MALDI-TOF MS analysis of salt-containing proteins".J. Am. Soc. Mass Spectrom. 16.9(2005):1488-1492.

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