Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip

	Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip
	Du Y(杜宇); Li N(李宁); Yang H(杨浩); Luo CH(罗春花); Gong YX; Tong CF(佟春芳); Gao YX(高宇新); Lv SQ(吕守芹); Long M(龙勉)
刊名	LAB ON A CHIP
	2017
卷号	17 期号:5 页码:782-794
ISSN号	1473-0197
中文摘要	Physiologically four major types of hepatic cells - the liver sinusoidal endothelial cells Kupffer cells hepatic stellate cells and hepatocytes - reside inside liver sinusoids and interact with flowing peripheral cells under blood flow. It is hard to mimic an in vivo liver sinusoid due to its complex multiple cell-cell interactions spatiotemporal construction and mechanical microenvironment. Here we developed an in vitro liver sinusoid chip by integrating the four types of primary murine hepatic cells into two adjacent fluid channels separated by a porous permeable membrane replicating liver's key structures and configurations. Each type of cells was identified with its respective markers and the assembled chip presented the liver-specific unique morphology of fenestration. The flow field in the liver chip was quantitatively analyzed by computational fluid dynamics simulations and particle tracking visualization tests. Intriguingly co-culture and shear flow enhance albumin secretion independently or cooperatively while shear flow alone enhances HGF production and CYP450 metabolism. Under lipopolysaccharide (LPS) stimulations the hepatic cell co-culture facilitated neutrophil recruitment in the liver chip. Thus this 3D-configured in vitro liver chip integrates the two key factors of shear flow and the four types of primary hepatic cells to replicate key structures hepatic functions and primary immune responses and provides a new in vitro model to investigate the short-duration hepatic cellular interactions under a microenvironment mimicking the physiology of a liver.
类目[WOS]	Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]	ON-A-CHIP ; ENDOTHELIAL-CELLS ; IN-VITRO ; DIFFERENTIAL REGULATION ; METABOLIC-ACTIVITY ; STEM-CELLS ; RAT-LIVER ; FLOW ; HEPATOCYTES ; MORPHOLOGY
收录类别	SCI
语种	英语
WOS记录号	WOS:000395893500003
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/60599]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Du Y,Li N,Yang H,et al. Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip[J]. LAB ON A CHIP,2017,17(5):782-794.
APA	杜宇.,李宁.,杨浩.,罗春花.,Gong YX.,...&龙勉.(2017).Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip.LAB ON A CHIP,17(5),782-794.
MLA	杜宇,et al."Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip".LAB ON A CHIP 17.5(2017):782-794.