Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction

	Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction
	Sun, Jing 1,2; Zhao, Xian-En 1,2,3,4; Dang, Jun 1,2; Sun, Xiaoyan 3,4; Zheng, Longfang 3,4; You, Jinmao 3,4; Wang, Xiao 5
刊名	journal of separation science
	2017-02-01
卷号	40 期号:3 页码:725-732
关键词	derivatization dispersive liquid-liquid microextraction phytosterol sensitivity enhancement triple quadrupole mass spectrometry
英文摘要	in this work, a hyphenated technique of dual ultrasound-assisted dispersive liquid-liquid microextraction combined with microwave-assisted derivatization followed by ultra high performance liquid chromatography tandem mass spectrometry has been developed for the determination of phytosterols in functional foods and medicinal herbs. multiple reaction monitoring mode was used for the tandem mass spectrometry detection. a mass spectrometry sensitive reagent, 4'-carboxy-substituted rosamine, has been used as the derivatization reagent for five phytosterols, and internal standard diosgenin was used for the first time. parameters for the dual microextraction, microwave-assisted derivatization, and ultra high performance liquid chromatography tandem mass spectrometry were all optimized in detail. satisfactory linearity, recovery, repeatability, accuracy and precision, absence of matrix effect, extremely low limits of detection (0.005-0.015 ng/ml) and limits of quantification (0.030-0.10 ng/ml) were achieved. the proposed methodwas compared with previously reported methods. it showed better sensitivity, selectivity, and accuracy. the matrix effect was also significantly reduced. the proposed method was successfully applied to the determination of five phytosterols in vegetable oil (sunflower oil, olive oil, corn oil, peanut oil), milk and orange juice (soymilk, peanut milk, orange juice), and medicinal herbs (ginseng, ganoderma lucidum, cordyceps, polygonum multiflorum) for the quality control of functional foods and medicinal herbs.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	tandem mass-spectrometry ; supercritical carbon-dioxide ; fluorescence detection ; chromatographic method ; phase microextraction ; gas-chromatography ; dairy-products ; water samples ; rhodamine-b ; extraction
收录类别	SCI
语种	英语
WOS记录号	WOS:000397029500013
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/6793]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining, Qinghai, Peoples R China 3.Qufu Normal Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Life Organ Anal, Qufu, Shandong, Peoples R China 4.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharm Intermediates & Anal Nat Med, Qufu, Shandong, Peoples R China 5.Shandong Acad Sci, Shandong Anal & Test Ctr, Key Lab TCM Qual Control Technol, Jinan, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jing,Zhao, Xian-En,Dang, Jun,et al. Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction[J]. journal of separation science,2017,40(3):725-732.
APA	Sun, Jing.,Zhao, Xian-En.,Dang, Jun.,Sun, Xiaoyan.,Zheng, Longfang.,...&Wang, Xiao.(2017).Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction.journal of separation science,40(3),725-732.
MLA	Sun, Jing,et al."Rapid and sensitive determination of phytosterols in functional foods and medicinal herbs by using UHPLC-MS/MS with microwave-assisted derivatization combined with dual ultrasound-assisted dispersive liquid-liquid microextraction".journal of separation science 40.3(2017):725-732.