基于WS2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究

CORC > 厦门大学 > 2017年第23卷

	基于WS2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究; Direct Electrochemistry of Glucose Oxidase Based on WS2 Quantum Dots and its Biosensing Application
	李晨露 ; 彭花萍 ; 黄种南 ; 盛依伦 ; 吴佩文 ; 林新华 ; LI Chen-lu ; PENG Hua-ping ; HUANG Zhong-nan ; SHENG Yi-lun ; WU Pei-wen ; LIN Xin-hua
刊名	http://electrochem.xmu.edu.cn/CN/abstract/abstract10316.shtml
	2017-02-28
关键词	WS2 量子点葡萄糖氧化酶直接电化学生物传感器 tungsten disulfide quantum dots glucose oxidase direct electrochemistry biosensor
英文摘要	采用水热法制备水溶性WS2量子点（WS2 QDs）材料，并将该材料进一步用于葡萄糖氧化酶（GOx）的有效固定，构建GOx/W2 QDs/GCE传感界面. 采用透射电镜、紫外-可见光谱和电化学等方法对材料的形貌、GOx的固定化过程，以及传感器的直接电化学和电催化性能进行了表征. 结果表明，WS2 QDs材料能够有效促进GOx与电极之间的直接电子转移. 并且，基于该传感器对葡萄糖良好的电催化作用，该方法有效实现了对葡萄糖的高灵敏检测，其线性范围为25 ~ 100 μmol·L-1和100 ~ 600 μmol·L-1，检测限为5.0 μmol·L-1（S/N=3）. 该传感器具有良好的选择性、重现性和稳定性，可用于实际样品血糖的分析测定.; In this study, a novel electrochemical glucose biosensor has been developed by immobilizing glucose oxidase (GOx) on tungsten disulfide quantum dots (WS2 QDs) on the surface of glassy carbon electrode (GCE). Transmission electron microscopy, UV-vis spectroscopy and cyclic voltammetry were employed to characterize the morphology, structure, and electrochemical behaviors of the as-prepared WS2 QDs and the biofilm modified electrode. The results suggested that the WS2 QDs could accelerate the electron transfer between the electrode and the immobilized enzyme, which enabled the direct electrochemistry of GOx without any electron mediator. Besides, the immobilized GOx in WS2 QDsfilm exhibited excellent electrocatalytic activity toward oxidation of glucose due to the excellent biocompatibility of the WS2 QDs. The proposed GOx/WS2 QDs biofilm electrode exhibited a linear response to glucose concentration in the ranges of 25 ~ 100 μmol·L-1 and 100 ~ 600 μmol·L-1, and the detection limit of the biosensor was as low as 5.0 μmol·L-1. The biosensor also exhibited good selectivity, reproducibility and long-term stability, which might be potenially used for the detection of the real samples.; 国家自然科学基金项目（No.21405015, No.81400805），福建省自然科学基金资助项目（No.2014J05092, No.2016J01449），大学生创新创业训练项目基金（No.C1634）资助; 作者联系地址：1. 福建医科大学附属第一医院高压氧室，福建福州 350005; 2. 福建医科大学药学院药物分析系，福建福州 350122; 3. 福建医科大学附属第一医院内分泌科，福建福州 350005; Author's Address: 1. Department of high pressure oxygen,, The Affiliated First Hospital, Fujian Medical University, Fuzhou 350005 , China;2. Department of Pharmaceutical Analysis of the Fujian Medical University，Fuzhou 350122，China；3. Department of Endocrinology, The Affiliated First Hospital, Fujian Medical University, Fuzhou 350005 , China; 通讯作者E-mail：phpfjmu@126.com, 247056892@qq.com
语种	中文
出版者	厦门大学《电化学》编辑部
内容类型	期刊论文
源URL	[http://dx.doi.org/10.13208/j.electrochem.160829]
专题	2017年第23卷
推荐引用方式 GB/T 7714	李晨露,彭花萍,黄种南,等. 基于WS2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究, Direct Electrochemistry of Glucose Oxidase Based on WS2 Quantum Dots and its Biosensing Application[J]. http://electrochem.xmu.edu.cn/CN/abstract/abstract10316.shtml,2017.
APA	李晨露.,彭花萍.,黄种南.,盛依伦.,吴佩文.,...&LIN Xin-hua.(2017).基于WS2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究.http://electrochem.xmu.edu.cn/CN/abstract/abstract10316.shtml.
MLA	李晨露,et al."基于WS2量子点材料固定葡萄糖氧化酶的直接电化学及其生物传感研究".http://electrochem.xmu.edu.cn/CN/abstract/abstract10316.shtml (2017).