基于电迁移率差异而分离的新型聚焦电泳技术

CORC > 厦门大学 > 1997年第3卷

	基于电迁移率差异而分离的新型聚焦电泳技术; A Novel Focusing Electrophoresis Techenique Based on the Mobility Differences
	陈东英 ; 林华水 ; 周勇亮 ; 毛秉伟 ; 谢兆雄 ; 卓向东 ; 田昭武 ; Chen Dongying ; Lin Huashui ; Zhou Yongliang ; Mao Bingwei ; Xie Zhaoxiong ; Zhuo Xiangdong ; Tian Zhaowu
刊名	http://electrochem.xmu.edu.cn/CN/abstract/abstract9572.shtml
	1997-05-28
关键词	Electrophoresis Focusing
英文摘要	基于电迁移率差异而分离的新型聚焦电泳技术①陈东英林华水周勇亮毛秉伟谢兆雄卓向东田昭武＊（固体表面物理化学国家重点实验室及化学系厦门大学厦门３６１００５）毛细管区带电泳已成为十几年来发展最快的分离分析技术．Ｈｊｅｒｔéｎ于１９６７年在玻璃管中进行了自由...; In this report, we present a novel analytical focusing electrophoresis technique. The principle of the technique is based on the mobility differences and has been proved successfully in our laboratory. The technique is of the same applicability as that of CZE, not limited to the protein like big molecules, with greatly improved efficiency of separation and enrichment. Advantages of this novel technique are:1. Since large volume of dilute sample solution can be injected and very narrow and concentrated zones can be obtained, extremely dilute sample can be analyzed by this novel technique in comparison with CZE. 2. Instead of with extremely small injection volume (nanoliters or less) in CZE, microliter quantities of sample can be injected in this novel technique. This quantity is similar to that in HPLC and is much easier to be handled. 3. Since species in a mixture are separated on the basis of their mobility differences, the field of application is as wide as that of CZE and is much wider than that of IEF (not restricted in proteins and no pH gradient is needed). 4. The separated narrow zones of different samples are stationary after focusing at different positions along the electrophoresis channel. Therefore, the length of the electrophoresis channel can be shortened to several centimeters and the applied voltage can thus be reduced to several hundreds of volts. This will facilitate the fabrication of miniaturized instrument and the optical detection system for an array of electrophoresis channels.; 作者联系地址：固体表面物理化学国家重点实验室及化学系; Author's Address: State Key Lab. for Phys. Chem. of Solid Surf. and Chem. Dept., Xiamen Univ., Xiamen 361005
语种	中文
出版者	厦门大学《电化学》编辑部
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/57315]
专题	1997年第3卷
推荐引用方式 GB/T 7714	陈东英,林华水,周勇亮,等. 基于电迁移率差异而分离的新型聚焦电泳技术, A Novel Focusing Electrophoresis Techenique Based on the Mobility Differences[J]. http://electrochem.xmu.edu.cn/CN/abstract/abstract9572.shtml,1997.
APA	陈东英.,林华水.,周勇亮.,毛秉伟.,谢兆雄.,...&Tian Zhaowu.(1997).基于电迁移率差异而分离的新型聚焦电泳技术.http://electrochem.xmu.edu.cn/CN/abstract/abstract9572.shtml.
MLA	陈东英,et al."基于电迁移率差异而分离的新型聚焦电泳技术".http://electrochem.xmu.edu.cn/CN/abstract/abstract9572.shtml (1997).