题名 | 三丁基锡和多氯联苯诱导褐菖鲉脑组织差异表达基因的分析及新基因NSP17功能的初步研究; Identification of differentially expressed genes in the brain of Sebastiscus marmoratus in response to tributyltin and polychlorinated biphenyls exposure and preliminary function analysis of a novel gene NSP17 |
作者 | 李博文 |
答辩日期 | 2011 ; 2009 |
导师 | 陈奕欣 |
关键词 | 三丁基锡 多氯联苯 褐菖鲉 差异表达基因 差异显示技术 基因功能研究 Tributyltin polychlorinated biphenyls Sebastiscus marmoratus 2-dimension electrophoresis subtracted suppressive hybridization annealing control primer-based differential display reverse transcription PCR novel gene research |
英文摘要 | 本实验以我国沿海常见的鱼类褐菖鲉(Sebastiscusmarmoratus)为主要研究对象,分别对三丁基锡(tributyltin,TBT)和多氯联苯(polychlorinatedbiphenyls,PCBs)急性暴露后鱼脑组织差异表达基因进行了筛选分析。采用的TBT浓度为500μg/Kg,PCBs浓度为3mg/Kg,通过腹腔注射对鱼体进行急性暴露,于暴露后3天取脑组织,分别利用抑制性差减杂交(suppressionPCRsubtractivehybridization,SSH)、基于引物退火控制技术的差异显示RT-PCR(AnnealControlPrimer-baseddifferen...; In this study, marine fish Sebastiscus marmoratus was adopted as experimental animal. 3 experimental approaches: 2-dimension electrophoresis system (2-DE), subtracted suppressive hybridization (SSH), and annealing control primer-based differential display reverse transcription PCR (ACP-ddRT-PCR) methodology, were employed to investigate differentially expressed genes in the brain of S. marmoratus ...; 学位:理学博士; 院系专业:生命科学学院生物学系_动物学; 学号:21620060153291 |
语种 | zh_CN |
出处 | http://210.34.4.13:8080/lunwen/detail.asp?serial=25043 |
内容类型 | 学位论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/46598] |
专题 | 生命科学-学位论文 |
推荐引用方式 GB/T 7714 | 李博文. 三丁基锡和多氯联苯诱导褐菖鲉脑组织差异表达基因的分析及新基因NSP17功能的初步研究, Identification of differentially expressed genes in the brain of Sebastiscus marmoratus in response to tributyltin and polychlorinated biphenyls exposure and preliminary function analysis of a novel gene NSP17[D]. 2011, 2009. |
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