| 题名 | Modeling, Simulation and Process Analysis of Two Enzyme Reactions in a Linked Cofactor Recycle; Modeling, Simulation and Process Analysis of Two Enzyme Reactions in a Linked Cofactor Recycle |
| 作者 | IMINABO JOHN TAMUNOSAKI |
| 答辩日期 | 2011 ; 2010 |
| 导师 | 陈秉辉 |
| 关键词 | NADPH COBE CHBE 建模 辅酶再生 过程分析 NADPH COBE CHBE modeling cofactor regeneration process analysis |
| 英文摘要 | 鉴于生物催化反应中的辅酶的高成本，有必要考虑其再生问题。论文工作包含模拟、建模、和建立一个用NADPH作辅酶，用ALR酶不对称还原COBE到CHBE的双酶反应体系关联的辅酶回收的分析过程。NADPH辅酶的再生是通过使用葡萄糖脱氢酶和NADP+把葡萄糖氧化成葡萄糖酸内酯来实现的。综合考虑几个反应参数并确定了经济上产量收益可行的每个参数的最佳值，这些参数的最优范围及最优值包括pH（6.3-7.2，6.3为最优），温度（26-32oC，27.5oC为最优）；辅酶比例（介于1.33和1.5之间的比率被确定为最高产品产量的比例范围），在80-130的酶比率也被确定为最佳的反应范围。通过模型分析，当COB...; The high cost of cofactors in biocatalytic reactions has made it necessary to consider its regeneration. This work involved the simulation; modeling and process analysis of a two-enzyme reaction in a linked cofactor recycle in the asymmetric reduction of COBE to CHBE using ALR enzyme and NADPH as a cofactor. The cofactor was regenerated through the oxidation of glucose to glucolactone using glucos...; 学位：工学硕士; 院系专业：化学化工学院化学工程与生物工程系_化学生物学专业; 学号：20420081153694 |
| 语种 | zh_CN |
| 出处 | http://210.34.4.13:8080/lunwen/detail.asp?serial=26287 |
| 内容类型 | 学位论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/33848]  |
| 专题 | 化学化工－学位论文 |
| 推荐引用方式 GB/T 7714 | IMINABO JOHN TAMUNOSAKI. Modeling, Simulation and Process Analysis of Two Enzyme Reactions in a Linked Cofactor Recycle, Modeling, Simulation and Process Analysis of Two Enzyme Reactions in a Linked Cofactor Recycle[D]. 2011, 2010. |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论