QQ客服 官方微博 反馈留言

	HPLC结合MALDI-TOF-MS分析木聚糖水解产物; HPLC and MALDI-Tof-MS Analysis of Hydrolysates of Xylan
	黎海龙 ; 李成国 ; 秦光辉 ; 刘健 ; 甘礼惠 ; 谢茹胜 ; 龙敏南
	2015-10-28
关键词	1-苯基-3-甲基-5-吡唑啉酮 低聚木糖 4-O-甲基葡萄糖醛酸 MALDI-TOF-MS HPLC 1-phenyl-3-methyl-5-pyrazolone xylo-oligosaccharides 4-O-methylglucuronic acid MALDI-Tof-MS HPLC
英文摘要	利用HPlC结合MAldI-TOf-MS对1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化后山毛榉木聚糖水解产物进行分析,检测到了难以获得标准品对照的木聚糖水解产物。结果表明,稀硫酸水解山毛榉木聚糖的主要水解产物有木糖和4-O-甲基葡萄糖醛酸-木糖(b2),以及少量4-O-甲基葡萄糖醛酸(b1)。内切重组木聚糖酶An Xyn10C水解山毛榉木聚糖产生木糖、木二糖和4-O-甲基葡萄糖醛酸-木三糖(b3),而内切重组木聚糖酶HO Xyn11A水解山毛榉木聚糖主要产生木糖、木二糖、木三糖、4-O-甲基葡萄糖醛酸-木四糖(b4)和4-O-甲基葡萄糖醛酸-木五糖(b5)。基于PMP柱前衍生化的HPlC结合MAldI-TOf-MS方法能高效地分析复杂的木聚糖水解产物。; The hydrolysis end products of beechwood xylan,which were released by sulfuric acid or enzymes and then labeled at their reducing ends with 1-phenyl-3-methyl-5-pyrazolone( PMP) derivatization,were analyzed by HPLC assisted with MALDITof-MS.Some of the hydrolysates,which were lack of related commercial available standard substances,were determined.It was found that the xylose and 4-O-methyl-glucuronic acid-xylose( B2) were the main products with minor amounts of 4-Omethylglucuronic acid( B1) in the hydrolysates of beechwood xylan by sulfuric acid.Recombinant endo-β-1,4-xylanase An Xyn10 C released xylose,xylobiose,and 4-O-methyl-glucuronic acid-xylotriose( B3) as the main hydrolysates from beechwood xylan,whereas recombinant endo-β-1,4-xylanase Ho Xyn11 A released xylose,xylobiose,xylotriose,4-O-methyl-glucuronic acidxylotetrose( B4) and-xylopentaose( B5),and aldohexaouronic acid.These results revealed that HPLC assisted with MALDITof-MS based on PMP derivatization was a very useful and robust method for the determination of products in hydrolysis of xylan.; 国家自然科学基金资助项目(31170067); 厦门市海洋经济发展专项资金项目(14GZP59HJ29); 福建省海洋高新产业发展专项项目(闽海洋高新[2014]25号)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/115475]
专题	能源院－已发表论文
推荐引用方式 GB/T 7714	黎海龙,李成国,秦光辉,等. HPLC结合MALDI-TOF-MS分析木聚糖水解产物, HPLC and MALDI-Tof-MS Analysis of Hydrolysates of Xylan[J],2015.
APA	黎海龙.,李成国.,秦光辉.,刘健.,甘礼惠.,...&龙敏南.(2015).HPLC结合MALDI-TOF-MS分析木聚糖水解产物..
MLA	黎海龙,et al."HPLC结合MALDI-TOF-MS分析木聚糖水解产物".(2015).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。