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	灰绿曲霉β-葡萄糖苷酶的分离及特性; Purification and Properties of β-glucosidase from Aspergillus glaucus EU7-22
	邓敏 ; 邬小兵 ; 李倩一 ; 黄丽春 ; 鲍思龙 ; 龙敏南
	2009
关键词	灰绿曲霉EU7-22 β-葡萄糖苷酶 分离纯化 理化特性 Aspergillus glaucus EU7-22 β-glucosidase purification property
英文摘要	目的:利用灰绿曲霉Eu7-22发酵产纤维素酶,从中分离到β-葡萄糖苷酶,分析其理化特性,确定其最佳活性条件。方法:灰绿曲霉Eu7-22发酵液离心后,上清液经硫酸铵沉淀、PHEnyl 6 fAST flOW(HIgHSub)疏水层析和SEPHACryl S-200凝胶层析,获得纯化的β-葡萄糖苷酶。结果:纯酶的比活性为5.1 Iu/Mg,得率为13.89%。SdS-PAgE凝胶电泳分析表明该酶是单亚基蛋白,其分子量为56.2 kdA。在PH4.0--6.0范围内,β-葡萄糖苷酶具有较高的稳定性,该酶的最适酶促反应PH为5.0。当β-葡萄糖苷酶在温度低于60℃的缓冲液中温育1 H后,酶活损失不大,表现了较好的稳定性;当该酶在温度高于60℃的缓冲液中温育1 H后,酶活迅速丧失。β-葡萄糖苷酶在70℃时具有最大催化活性。结论:灰绿曲霉Eu7-22发酵产生的β-葡萄糖苷酶具有较高活性,具有分子量较小、最佳催化温度较高的特点。; Objective:β-glucosidase produced by Aspergillus glaucus EU7-22 was purified and characterized.Method:The β-glucosidase of Aspergillus glaucus EU722 was purified from ferment supernatant liquid by three steps of purification,ammonium sulfate precipitation(80%,W/V),Phenyl 6 Fast Flow(high sub) column chromatography and Sephacryl S-200 column chromatography,with a specific activity of 5.1 IU/mg and a yield of 13.89%.Result:The purified β-glucosidase was determined as a monomeric protein with a molecular weight of 56.2 kDa.The enzyme exhibited high stability when it was kept in the buffer at pH 4.0～6.0 and at the temperature below 60℃.β-glucosidase exhibited its optimal activity at 70℃.Conclusion: β-glucosidase produced by Aspergillus glaucus EU7-22 with small molecular showed high activity under the optimal conditions.; 国家“973”计划项目(2010CB732201);国际科技合作重点项目(2009DFA60930)资助
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/115424]
专题	能源院－已发表论文
推荐引用方式 GB/T 7714	邓敏,邬小兵,李倩一,等. 灰绿曲霉β-葡萄糖苷酶的分离及特性, Purification and Properties of β-glucosidase from Aspergillus glaucus EU7-22[J],2009.
APA	邓敏,邬小兵,李倩一,黄丽春,鲍思龙,&龙敏南.(2009).灰绿曲霉β-葡萄糖苷酶的分离及特性..
MLA	邓敏,et al."灰绿曲霉β-葡萄糖苷酶的分离及特性".(2009).

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