| 补镁对2型糖尿病大鼠胰岛素受体表达水平的影响; Effects of magnesium intake on expression of insulin receptor in type 2 diabetes rats | |
| Cong PD(丛朋地) ; Zhong JY(钟进义) ; Zhang J(张静) ; Sun JP(孙健平) ; Li L(李蕾) | |
| 2012 | |
| 关键词 | 镁 2型糖尿病 胰岛素受体 magnesium type 2 diabetes mellitus insluin receptor |
| 英文摘要 | 目的观察补镁对2型糖尿病大鼠胰岛素受体(InSulIn rECEPTOr,Ir)表达水平的影响。方法将高脂饲料喂养加链脲佐菌素注射方法诱发的2型糖尿病大鼠随机分为4个组,高、中、低剂量组在高脂饲料中分别加入氧化镁2000、1000、200Mg/kg(以镁离子计),糖尿病对照组只喂饲高脂饲料,正常对照组喂饲普通饲料。动物自由进食,连续4周,用葡萄糖氧化酶法测定空腹尾尖血的血糖含量,腹主动脉取血用放免法测定血清胰岛素含量,用免疫组化法测定胰腺和骨骼肌组织Ir表达水平。结果经IMAgE-PrO PluS图像分析,高剂量组的胰腺和骨骼肌组织中Ir表达水平分别为0.341±0.001和0.346±0.002,均较糖尿病对照组升高,而血糖水平则较糖尿病对照组降低,差异均有显著性意义(P<0.05)。结论镁补充可以提高2型糖尿病大鼠胰腺和骨骼肌组织中Ir的表达水平,降低空腹血糖水平。; Objective To investigate the effects of magnesium intake on expression of insulin receptor in type 2 diabetes rats.Methods The models of type 2 diabetes rats were established by feeding with high-fat-diet and injecting streptozotocin(STZ).Rats were randomly assigned to four groups.The high-fat-diets were administrated magnesium at a dose of 2000(high magnesium group),1000(medium magnesium group)and200(low magnesium group).Model control group was only fed with high-fat-diet.Normal control group was fed with common diet.Rats ate freely for four weeks.Fasting blood glucose was detected by glucose oxidase method..Insulin was detected by radio-immunity method.The expression levels of IR of pancreas and skeletal muscle were detected by immunohistochemistry method.Results The immunohistochemistry pictures were analyzed by Image-Pro Plus.Compared with model control group,the expression levels of IR of pancreas and skeletal muscle were 0.341±0.001 and 0.346±0.002,increased and fasting blood glucose decreased significantly in high magnesium group(P<0.05).Conclusion Magnesium intake may increase the expression levels of IR of pancreas and skeletal muscle,and decrease fasting blood glucose in type 2 diabetes rats. |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/102208]  |
| 专题 | 公共卫生－已发表论文 |
| 推荐引用方式 GB/T 7714 | 丛朋地,钟进义,张静,等. 补镁对2型糖尿病大鼠胰岛素受体表达水平的影响, Effects of magnesium intake on expression of insulin receptor in type 2 diabetes rats[J],2012. |
| APA | 丛朋地,钟进义,张静,孙健平,&李蕾.(2012).补镁对2型糖尿病大鼠胰岛素受体表达水平的影响.. |
| MLA | 丛朋地,et al."补镁对2型糖尿病大鼠胰岛素受体表达水平的影响".(2012). |
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