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	补充镁对2型糖尿病大鼠胰岛素抵抗和受体亲和力的影响; INFLUENCE of MAGNESIUM SUPPLEMENTATION ON INSULIN RESISTANCE AND THE INFINITY of RECEPTOR IN RATS WITH TYPE 2 DIABETES MELLITUS
	张静 ; 姜秀波 ; 丛朋地 ; 孙健平 ; 李蕾 ; 钟进义
	2012
关键词	镁 糖尿病 胰岛素受体 胰岛素受体亲和力 magnesium insulin receptor the infinity of insulin receptor insulin resistance diabetes mellitus
英文摘要	目的观察镁补充对2型糖尿病大鼠胰岛素受体亲和力的影响。方法将用高脂饮食联合链脲佐菌素(STz)方法诱发的2型糖尿病大鼠随机分为4个组,糖尿病对照组喂饲高脂饲料,高、中、低剂量组在高脂饲料中分别加入2000、1000、200Mg/kg的镁(以镁离子计)。每周检测空腹血糖1次。自由饮食喂养4 W,处死动物。用放射性受体分析法测肝细胞胰岛素受体亲和力、放射免疫法测血清胰岛素(InS)水平、比色法检测糖化血红蛋白(HbA1C)和血浆丙二醛(MdA)、葡萄糖氧化酶法测空腹血浆葡萄糖(fPg),并计算胰岛素敏感指数(ISI)和抵抗指数(IrI)。结果高剂量组的高亲和力胰岛素受体结合常数(k1)为(4.76±0.08)x108l/MOl,低亲和力胰岛素受体结合常数(k2)与结合容量(r2)分别为(1.10±0.14)x106l/MOl,(8.49±0.43)x1013/Mg蛋白,均较糖尿病对照组显著性升高(P<0.05)。补镁第3 W开始高剂量组空腹血糖较糖尿病对照组显著性降低,高剂量组的胰岛素敏感指数较糖尿病对照组显著性升高,而胰岛素抵抗指数则显著性降低。结论镁补充可以提高2型糖尿病大鼠胰岛素受体亲和力,降低胰岛素抵抗。; Objective To observe the influence of magnesium supplementation on the infinity of insulin receptor in rats with type 2 diabetes mellitus.Method The rats with type 2 diabetes mellitus were induced with high fat diet and intraperitoneal injection with small dose of STZ.They were randomly divided into 4 groups,i.e.high,medium,low magnesium(2000,1000,200 mg/kg Mg2+) supplementation groups and diabetes mellitus control group.FPG was measured weekly.All rats were killed after 4 w,and liver cells were tested with radiation receptor analysis of their combination constants and combined capacity.The serum insulin(Ins) level was determined with radioimmunoassay.FPG,glycosylated hemoglobin(HbA1c) and plasma malondialdehyde(MDA) were also measured,and insulin resistance index(IRI) and insulin sensitivity index(ISI) were calculated.Results: High affinity insulin receptor combination constant(K1),low affinity insulin receptor combination constant(K2) and combination capacity(R2) of high dosage group were(4.76±0.08)×108 L/ mol,(1.10±0.14)×106 L/ mol,(8.49±0.43)×1013 /mg protein respectively,significantly increased than those in diabetes mellitus control group(P<0.05).FPG was decreased since the 3th week during magnesium supplementation.The insulin sensitivity index(ISI) of high dosage group was significantly increased than that of model control group,but insulin resistance index(IRI) was decreased.Conclusion Magnesium supplementation could improve insulin receptor affinity and reduce insulin resistance in type 2 diabetic rats.
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/102206]
专题	公共卫生－已发表论文
推荐引用方式 GB/T 7714	张静,姜秀波,丛朋地,等. 补充镁对2型糖尿病大鼠胰岛素抵抗和受体亲和力的影响, INFLUENCE of MAGNESIUM SUPPLEMENTATION ON INSULIN RESISTANCE AND THE INFINITY of RECEPTOR IN RATS WITH TYPE 2 DIABETES MELLITUS[J],2012.
APA	张静,姜秀波,丛朋地,孙健平,李蕾,&钟进义.(2012).补充镁对2型糖尿病大鼠胰岛素抵抗和受体亲和力的影响..
MLA	张静,et al."补充镁对2型糖尿病大鼠胰岛素抵抗和受体亲和力的影响".(2012).

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