| 植物多酚清除自由基活性高通量筛选技术研究; Study on the free radical scavenging activity of polyphenolic compounds by on-line high-flux screening method | |
| 柳艳 ; 傅博强 ; 李磊 ; 戴金凤 ; 赵鸿雁 | |
| 2009 | |
| 关键词 | 植物化学物 HPLC-DPPH 自由基清除 流动注射 phytochemicals HPLC-DPPH free radicals scavenging flow injection |
| 英文摘要 | 目的:构建植物化学物在线清除自由基活性高通量筛选技术,创建植物化学物抗氧化活性研究新方法。方法:以附带2个检测器的高效液相色谱(HPlC)装置和流动注射技术为手段,引入二苯基三硝基苯肼(dPPH)有机自由基分别与经HPlC分离的植物化学物发生在线清除反应,优化流速、反应管尺寸等条件,比较反应前后dPPH吸收信号的变化。结果:确定和验证了两类植物多酚化学物茶多酚和丹酚酸在线清除自由基活性HPlC-dPPH表征方法。反应环聚醚醚酮(PEEk)盘管规格为长10M、内径0.254MM,dPPH·浓度为1.0x10-5MOl/l,流速为0.7~0.8Ml/MIn。结论:HPlC-dPPH流动注射在线表征技术能够实现对多组分植物化学物抗氧化能力的高通量筛选。; Objective:To construct a high-flux screening procedure by on-line free radical scavenging activity method of phyto- chemicals, and create a new evaluation system of antioxidant activity of natural plant materials.Methods:A HPLC system with two detectors and flow injection techniques was employed.An organic free radicals DPPH was induced on-line HPLC-DPPH and reaction with the single phytochemical components isolated by HPLC.To optimize reactive conditions including flow rate and the size of reaction coil, and detect the DPPH signals before and after reaction.Results:A new on-line HPLC-DPPH method was established for a high-flux screening of several free radical scavenging components exampled as Tea polyphenols and Salvianolic acids.The reaction coil of PEEK was 10 m length and inner diameter 0.254 mm, the concentration and flow velocity of DPPH was 1.0 × 10-5mol/L and 0.7～0.8 ml/ min, respectively.Conclusion:The PHLC-DPPH flow injection hyphenated technique could realize high-flux screening analysis for antioxidant of phytochemicals.; 江苏省高校自然科学研究计划资助项目(05KJB330083) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/102190]  |
| 专题 | 公共卫生－已发表论文 |
| 推荐引用方式 GB/T 7714 | 柳艳,傅博强,李磊,等. 植物多酚清除自由基活性高通量筛选技术研究, Study on the free radical scavenging activity of polyphenolic compounds by on-line high-flux screening method[J],2009. |
| APA | 柳艳,傅博强,李磊,戴金凤,&赵鸿雁.(2009).植物多酚清除自由基活性高通量筛选技术研究.. |
| MLA | 柳艳,et al."植物多酚清除自由基活性高通量筛选技术研究".(2009). |
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