Real-time monitoring of caspase cascade activation in living cells

CORC > 厦门大学 > 公共卫生－已发表论文

	Real-time monitoring of caspase cascade activation in living cells
	Zhu, Lei ; Huang, Xinglu ; Choi, Ki Young ; Ma, Ying ; Zhang, F ; Liu, Gang ; Lee, Seulki ; Chen, Xiaoyuan ; Liu G(刘刚) ; Chen XY(陈小元)
刊名	http://dx.doi.org/10.1016/j.jconrel.2012.05.044
	2012
关键词	Cell death Drug products Enzyme activity Peptides Probes
英文摘要	We introduce a simple, versatile and robust one-step technique that enables real-time imaging of multiple intracellular caspase activities in living cells without the need for complicated synthetic protocols. Conventional fluorogenic probes or recently reported activatable probes have been designed to target various proteases but are limited to extracellular molecules. Only a few have been applied to image intracellular proteases in living cells because most of these probes have limited cell-permeability. Our platform does not need complicated synthetic processes; instead it involves a straightforward peptide synthesis and a simple mixing step with a commercial transfection agent. The transfection agent efficiently delivered the highly quenched fluorogenic probes, comprised of distinctive pairs of dyes and quenchers, to the initiator caspase-8 and the effector caspase-3 in MDA-MB-435 cells, allowing dual-imaging of the activities of both caspases during the apoptotic process induced by TNF-related apoptosis induced ligand (TRAIL). With the combination of multiple fluorogenic probes, this simple platform can be applied to multiplexed imaging of selected intracellular proteases to study apoptotic processes in pathologies or for cell-based high throughput screening systems for drug discovery.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87601]
专题	公共卫生－已发表论文
推荐引用方式 GB/T 7714	Zhu, Lei,Huang, Xinglu,Choi, Ki Young,et al. Real-time monitoring of caspase cascade activation in living cells[J]. http://dx.doi.org/10.1016/j.jconrel.2012.05.044,2012.
APA	Zhu, Lei.,Huang, Xinglu.,Choi, Ki Young.,Ma, Ying.,Zhang, F.,...&陈小元.(2012).Real-time monitoring of caspase cascade activation in living cells.http://dx.doi.org/10.1016/j.jconrel.2012.05.044.
MLA	Zhu, Lei,et al."Real-time monitoring of caspase cascade activation in living cells".http://dx.doi.org/10.1016/j.jconrel.2012.05.044 (2012).