Characterization of virus-like particles in GARDASIL (R) by cryo transmission electron microscopy

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	Characterization of virus-like particles in GARDASIL (R) by cryo transmission electron microscopy
	Zhao, Qinjian ; Potter, Clinton S. ; Carragher, Bridget ; Lander, Gabriel ; Sworen, Jaime ; Towne, Victoria ; Abraham, Dicky ; Duncan, Paul ; Washabaugh, Michael W. ; Sitrin, Robert D. ; Zhao QJ(赵勤俭)
刊名	http://dx.doi.org/10.4161/hv.27316
	2014
关键词	PAPILLOMAVIRUS TYPE 11 MAJOR CAPSID PROTEIN MONOCLONAL-ANTIBODIES CONFORMATIONAL EPITOPES BOVINE PAPILLOMAVIRUS ANGSTROM RESOLUTION THERMAL-STABILITY L1 PROTEIN IN-VITRO RECONSTRUCTION
英文摘要	Chinese National Science Fund [81273327]; 863 Major Project [2012AA02A408]; Cryo-transmission electron microscopy (cryoTEM) is a powerful characterization method for assessing the structural properties of biopharmaceutical nanoparticles, including Virus Like Particle-based vaccines. We demonstrate the method using the Human Papilloma Virus (HPV) VLPs in GARDASIL (R). CryoTEM, coupled to automated data collection and analysis, was used to acquire images of the particles in their hydrated state, determine their morphological characteristics, and confirm the integrity of the particles when absorbed to aluminum adjuvant. In addition, we determined the three-dimensional structure of the VLPs, both alone and when interacting with neutralizing antibodies. Two modes of binding of two different neutralizing antibodies were apparent; for HPV type 11 saturated with H11.B2, 72 potential Fab binding sites were observed at the center of each capsomer, whereas for HPV 16 interacting with H16.V5, it appears that 60 pentamers (each neighboring 6 other pentamers) bind five Fabs per pentamer, for the total of 300 potential Fab binding sites per VLP.
语种	英语
出版者	LANDES BIOSCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87585]
专题	公共卫生－已发表论文
推荐引用方式 GB/T 7714	Zhao, Qinjian,Potter, Clinton S.,Carragher, Bridget,et al. Characterization of virus-like particles in GARDASIL (R) by cryo transmission electron microscopy[J]. http://dx.doi.org/10.4161/hv.27316,2014.
APA	Zhao, Qinjian.,Potter, Clinton S..,Carragher, Bridget.,Lander, Gabriel.,Sworen, Jaime.,...&赵勤俭.(2014).Characterization of virus-like particles in GARDASIL (R) by cryo transmission electron microscopy.http://dx.doi.org/10.4161/hv.27316.
MLA	Zhao, Qinjian,et al."Characterization of virus-like particles in GARDASIL (R) by cryo transmission electron microscopy".http://dx.doi.org/10.4161/hv.27316 (2014).