Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death

CORC > 厦门大学 > 生物医学－已发表论文

	Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death
	Xu, Yang ; Wang, Guanghui ; Chen, Quancheng ; Lin, Ting ; Zeng, Zhiping ; Luo, Qiang ; Liu, Jie ; Sun, Cuiling ; Xu Y(徐阳) ; Wang GH(王光辉) ; Chen QC(陈全成) ; Lin T(林挺) ; Ceng ZP(曾志平)
刊名	http://dx.doi.org/10.3978/j.issn.1000-9604.2013.06.03
	2013
关键词	NUCLEAR RIBONUCLEOPROTEIN K CANCER-CELLS DNA-DAMAGE MITOCHONDRIAL TRANSLATION ESOPHAGEAL CANCER PROHIBITIN P53 PROLIFERATION INTERFERENCE SUMOYLATION
英文摘要	Natural Science Foundation of Fujian Province of China [2011J05098]; Fundamental Research Funds for the Central Universities [2011121055]; National Natural Science Foundation of China [81202956]; SRF for ROCS, SEM [[2011]1568]; NSFC [81102332]; Objective: Squamous esophageal carcinoma is highly prevalent in developing countries, especially in China. Tu Bei Mu (TBM), a traditional folk medicine, has been used to treat esophageal squamous cell carcinoma (ESCC) for a long term. tubeimoside I (TBMS1) is the main component of TBM, exhibiting great anticancer potential. In this study, we investigated the mechanism of TBMS1 cytotoxic effect on EC109 cells. Methods: Comparative nuclear proteomic approach was applied in the current study and we identified several altered protein spots. Further biochemical studies were carried out to detect the mitochondrial membrane potential, cell cycle and corresponding proteins' expression and location. Results: Subcellular proteomic study in the nucleus from EC109 cells revealed that altered proteins were associated with mitochondrial function and cell proliferation. Further biochemical studies showed that TBMS1-induced molecular events were related to mitochondria-induced intrinsic apoptosis and P21-cyclin B1/cdc2 complex-related G2/M cell cycle arrest. Conclusions: Considering the conventional application of TBM in esophageal cancer, TBMS1 therefore may have a great potential as a chemotherapeutic drug candidate for ESCC.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90929]
专题	生物医学－已发表论文
推荐引用方式 GB/T 7714	Xu, Yang,Wang, Guanghui,Chen, Quancheng,et al. Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death[J]. http://dx.doi.org/10.3978/j.issn.1000-9604.2013.06.03,2013.
APA	Xu, Yang.,Wang, Guanghui.,Chen, Quancheng.,Lin, Ting.,Zeng, Zhiping.,...&曾志平.(2013).Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death.http://dx.doi.org/10.3978/j.issn.1000-9604.2013.06.03.
MLA	Xu, Yang,et al."Intrinsic apoptotic pathway and G2/M cell cycle arrest involved in tubeimoside I-induced EC109 cell death".http://dx.doi.org/10.3978/j.issn.1000-9604.2013.06.03 (2013).