The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells

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	The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells
	Do Thi Ha ; Trinh Nam Trung ; Tran Thi Phuong ; Yim, Namhui ; Chen, Quan Cheng ; Bae, KiHwan ; Chen QC(陈全成)
刊名	http://dx.doi.org/10.1016/j.bmcl.2010.08.054
	2010-10-15
关键词	SYNTHASE GENE-TRANSCRIPTION PROTEIN-KINASE ADIPOSE-TISSUE JAPONICA L. PPAR-GAMMA ADIPOGENESIS ACID IDENTIFICATION QUERCETIN OBESITY
英文摘要	Ministry of Education, Science and Technology [KOSEF2009-007-4808]; Among nine flavonols (1-9) obtained from Sophorae Flos, we first isolated compounds 4, 5, 8, and 9. These isolates (1-9) were evaluated for the phosphorylation of AMPK and ACC. Administered at 10 mu M, 9 possessed high potent activity. Compound 9 displayed a dose-dependent stimulation of glucose uptake in 3T3-L1 cells, and this increase was obviously attenuated by compound C, an AMPK inhibitor. In addition, 9 also phosphorylated AMPK and its downstream substrate ACC in 3T3-L1 cells in a time-and dose-dependent manner. Moreover, we discovered that compound C inhibits 9-stimulated ACC phosphorylation and motivated the 9-inhibited C/EBP alpha and PPAR gamma, and FAS gene expression, significantly. These results revealed the role of the AMPK downstream signaling pathway in 9-improved glucose metabolism in 3T3-L1 cells and 9-inhibited adipocyte differentiation. Differentiation was investigated by Oil Red O staining activity after 9 administration (0-20 mu M) in 6 days. Compound 9 decreased mean droplet size in a dose-dependent manner. The results revealed that 9 blocked adipogenic conversion in 3T3-L1 cells together with several significant downregulating adipocyte-specific transcription factors, including PPAR gamma, C/EBP alpha, and SREBP1. It also reduced FAS gene expression in a dose-dependent manner, which is crucial for adipogenesis in vitro. (C) 2010 Elsevier Ltd. All rights reserved.
语种	英语
出版者	BIOORG MED CHEM LETT
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90918]
专题	生物医学－已发表论文
推荐引用方式 GB/T 7714	Do Thi Ha,Trinh Nam Trung,Tran Thi Phuong,et al. The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells[J]. http://dx.doi.org/10.1016/j.bmcl.2010.08.054,2010.
APA	Do Thi Ha.,Trinh Nam Trung.,Tran Thi Phuong.,Yim, Namhui.,Chen, Quan Cheng.,...&陈全成.(2010).The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells.http://dx.doi.org/10.1016/j.bmcl.2010.08.054.
MLA	Do Thi Ha,et al."The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells".http://dx.doi.org/10.1016/j.bmcl.2010.08.054 (2010).