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	芒针治疗对脊髓损伤大鼠TNF-α、IL-6β、IL-1β、NF-Kβ表达的影响; Effects of elongated needle on the expression of TNF-α, IL-6β, IL-1β and NF-Kβ in rats with spinal cord injury
	全仁夫 ; 李长明 ; 谢尚举 ; 王亚东 ; 杨宗保
	2015-10-10
关键词	芒针 脊髓损伤 肿瘤坏死因子α 白介素-6 白介素-1β 核转录因子Kβ Elongated needle Spinal cord injury TNF-α IL-6 IL-1β NF-Kβ
英文摘要	目的:应用改良AllEn’S造模法制备大鼠急性脊髓损伤(SCI)模型,观察芒针对大鼠急性脊髓损伤(ASCI)后脊髓组织中肿瘤坏死因子α(Tnf-α)、白介素-6(Il-6),白介素-1(Il-1β)和核转录因子kβ(nf-kβ)含量的影响。方法:将72只Sd大鼠随机分为两组:模型组36只和芒针组36只,采用改良AllEn’S打击法致伤模型组和芒针组大鼠T9~T11脊髓。芒针组采用芒针刺秩边与水道穴,1次/d,共治疗7d。分别于术后1d,3d,7d行bbb评分测定;术后1d,3d,7d取材,用EllSA法检测脊髓组织中Tnf-α、Il-6、Il-1β、nf-kβ的含量。结果:实验发现7d时芒针组bbb评分明显高于模型组,差异具有统计学意义(P<0.05);脊髓损伤后,模型组和芒针治疗组Tnf-α、Il-6、Il-1β、nf-kβ含量显著升高。经治疗,与模型组相比,芒针组受损脊髓组织内Tnf-α,Il-6、Il-1β、nf-kβ的表达均降低,且在1d,3d,7d的差异具有统计学意义(P<0.01)。结论:早期芒针治疗可显著抑制脊髓损伤炎性因子Tnf-α、Il-6、Il-1β、nf-kβ的表达,改善局部微环境,促进损伤后期大鼠运动功能的恢复。; Objective: By SCI model, effects of elongated needle on TNF-α, IL-1β, IL-6 and NF-Kβ were observed.Methods: 72 SD rats are randomly divided into the model group(n=36) and the elongated needle treatment group(n=36).T9~T11 spinal cord injury model is created by Allen's method.The elongated needle group took Zhi Bian and Shuidao acupoint.BBB scores were invested on 1d, 3d, 7d after injury.The expression of TNF-α? IL-6? IL-1β and NF-K β in the injuryed spinal cords are examined by ELISA method at 1d, 3d, 7d after injury.Results: In 7 days, BBB scores in the treatment group was higher(P<0.05).The expression of TNF-α, IL-6? IL-1β and NF-Kβ in two groups were significantly increased after injuried.The expression of TNF-α, IL-6? IL-1β and NF-Kβ of elongated needle group was significantly lower than that in the model group; in 1d, 3d, 7d, the difference was statistically significant(P<0.01).Conclusion: Elongated needle treatment can effectively inhibit the expression of pro-inflammatory cytokine TNF-α, IL-6? IL-1β and NF-Kβ in rats of acute spinal cord injuries, improve the micro-environment, and promote functional recovery of acute spinal cord injury rats.; 浙江省中医药科研计划项目(2008CB067)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/127249]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	全仁夫,李长明,谢尚举,等. 芒针治疗对脊髓损伤大鼠TNF-α、IL-6β、IL-1β、NF-Kβ表达的影响, Effects of elongated needle on the expression of TNF-α, IL-6β, IL-1β and NF-Kβ in rats with spinal cord injury[J],2015.
APA	全仁夫,李长明,谢尚举,王亚东,&杨宗保.(2015).芒针治疗对脊髓损伤大鼠TNF-α、IL-6β、IL-1β、NF-Kβ表达的影响..
MLA	全仁夫,et al."芒针治疗对脊髓损伤大鼠TNF-α、IL-6β、IL-1β、NF-Kβ表达的影响".(2015).

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