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	TNFAIP8干扰质粒的构建及最佳干扰效率质粒的筛选; Construction and identification of interference plasmid targeting on TNFAIP8
	Liu WM(刘文明) ; Yang JJ(杨晶晶) ; Hu RY(胡如意) ; Qiu XF(邱兴烽) ; Shi CY(石春燕) ; Qi ZQ(齐忠权) ; Liu ZC(刘忠臣) ; Zhuang GH(庄国洪)
	2015-05-20
关键词	TNFAIP8 RNA干扰 重组质粒 筛选 TNFAIP8 RNA interference Recombinant plasmid Screen
英文摘要	目的:构建并筛选出干扰效率最佳的TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒。方法:通过生物软件选择3个TnfAIP8基因干扰位点,构建干扰质粒并测序验证,将干扰质粒及对照质粒分别转染至A549细胞,通过rT-PCr、WESTErn blOT检测干扰效率。结果:经rT-PCr和WESTErn-blOT证实TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒能有效干扰并抑制细胞内TnfAIP8基因的表达,通过流式检测发现降低TnVAIP8表达可以提高细胞对A dr5SC fV诱导凋亡的敏感性。结论:成功构建和设计了对TnfAIP8基因具有显著干扰效率的干扰质粒,为进一步研究TnfAIP8基因的功能奠定了基础。; Objective: To construct and screen the high efficiency interference plasmid of TFAIP8-shRNA-p SIRENRetro Q.Methods: Selected and synthesized three Target Sequence of TNFAIP8 shRNA1,TNFAIP8 shRNA2,TNFAIP8 shRNA3,and construct the TNFAIP8 interference plasmid.Transfection TNFAIP8-shRNA-p SIREN-Retro Q interference plasmid to A549 cells.Filter out the highest interference efficiency plasmid by detecting the mRNA and protein levels using RT-PCR and Western blot methods.Results: We successfully design and built three TNFAIP8-shRNA-p SIREN-Retro Q interference plasmids,and screen out the highest efficiency interference plasmid.Conclusion: Three interference plasmids targeting the TNFAIP8 gene have been constructed successfully and provide a useful tool for studying the function of TNFAIP8.; 国家自然科学基金项目(81272720); 福建省卫计委医学创新课题(2014-CXB-43); 厦门市科技计划项目(3502Z2083008)资助
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/123776]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	刘文明,杨晶晶,胡如意,等. TNFAIP8干扰质粒的构建及最佳干扰效率质粒的筛选, Construction and identification of interference plasmid targeting on TNFAIP8[J],2015.
APA	刘文明.,杨晶晶.,胡如意.,邱兴烽.,石春燕.,...&庄国洪.(2015).TNFAIP8干扰质粒的构建及最佳干扰效率质粒的筛选..
MLA	刘文明,et al."TNFAIP8干扰质粒的构建及最佳干扰效率质粒的筛选".(2015).

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