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	EGFR参与溶血磷脂酸诱导口腔鳞癌细胞整合素β6的表达; EGFR Is Involved in Lysophosphatidic Acid Induced Integrinβ6 Expression in Oral Squamous Carcinoma Cells
	殷豪 ; 邓小玲 ; 李国阳 ; 石松林 ; 李祺福 ; 许铭炎
	2015-08-28
关键词	溶血磷脂酸 表皮生长因子受体 整合素β6 口腔鳞癌 Lysophosphatidic acid EGFR ITGB6 Oral squamous carcinoma
英文摘要	目的:探讨表皮生长因子受体(Egfr)在溶血磷脂酸(lPA)诱导口腔鳞癌细胞整合素β6(ITgb6)表达中的作用。方法:将口腔鳞癌细胞SAS接种到6孔板中,经lPA诱导后,收集细胞,用定量rT-PCr和WESTErn blOT检测ITgb6的MrnA和蛋白表达量;利用Egfr抑制剂Ag1478检测Egfr对lPA诱导ITgb6MrnA和蛋白表达的影响。结果:1)经10μMOl/l lPA诱导2H后,SAS细胞ITgb6的MrnA表达水平显著性增加;而ITgb6的蛋白水平则在诱导4H后有显著性升高。2)lPA诱导促进SAS细胞Egfr磷酸化水平增加。3)Egfr抑制剂Ag1478抑制lPA诱导SAS细胞ITgb6MrnA和蛋白表达。结论:lPA能诱导SAS口腔鳞癌细胞ITgb6MrnA和蛋白表达,Egfr参与lPA诱导的ITgb6表达。; Objective:To investigate the role of EGFR in the process of integrinβ6(ITGB6)expression in oral squamous carcinoma(SAS)cells induced by lysophosphatidic acid(LPA).Methods:SAS cells were cultured in 6-well culture plate and stimulated with 10μmol/L LPA.The cells were collected for RNA and protein extraction.The mRNA and protein level of ITGB6 were detected by real-time RT-PCR and Western blotting,respectively.The EGFR-specific inhibitor AG1487 was applied to investigate the role of EGFR activation in LPA induced ITGB6 expression.Results:The mRNA level of ITGB6 in SAS cells was statistically upregulated after treated with LPA for2 h,while the protein expression of ITGB6 was obviously increased after incubation with LPA for 4h.The increased phosphorylation of the EGFR was induced by LPA stimulation in SAS cells.EGFR inhibitor AG1478 could block LPA induced ITGB6 mRNA and protein expression in SAS cells.Conclusion:LPA can induce the ITGB6 expression in the oral SAS cells,and EGFR transactivation may be involved in this process.; 国家自然科学基金项目(81072208;30900661;81370160); 福建省卫生系统中青年骨干人才培养项目(2015-ZQN-ZD-35); 福建省医学创新课题(2014-CXB-47); 中央高校基本业务费专项资金资助; 厦门市科技计划项目(3502Z20154072)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/123773]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	殷豪,邓小玲,李国阳,等. EGFR参与溶血磷脂酸诱导口腔鳞癌细胞整合素β6的表达, EGFR Is Involved in Lysophosphatidic Acid Induced Integrinβ6 Expression in Oral Squamous Carcinoma Cells[J],2015.
APA	殷豪,邓小玲,李国阳,石松林,李祺福,&许铭炎.(2015).EGFR参与溶血磷脂酸诱导口腔鳞癌细胞整合素β6的表达..
MLA	殷豪,et al."EGFR参与溶血磷脂酸诱导口腔鳞癌细胞整合素β6的表达".(2015).

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