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	针刺调节急性胃黏膜损伤大鼠胃黏膜细胞增殖信号通路相关分子的表达; Expression of the proliferation signaling pathway on the gastric mucosa cells in rats with acute gastric mucosa injury treated by acupuncture
	王晨光 ; 杨宗保 ; 左彬荣 ; 谢宇峰 ; 刘琼
	2014-09-01
关键词	针刺 胃黏膜损伤 MAPK信号通路 PI3K/Akt-PAK1信号通路 Acupuncture Gastric mucosa injury MAPK signaling pathway PI3K/Akt-PAK1 signaling pathway
英文摘要	目的:研究针刺对急性胃黏膜损伤大鼠胃黏膜细胞增殖相关信号分子表达的影响。方法:大鼠随机分为空白组、模型组、胃经穴组和对照点组,采用乙醇灌胃法制作胃黏膜损伤大鼠模型,肉眼下观察大鼠胃黏膜损伤指数,酶联免疫吸附测定法(ElISA法)检测丝裂原激活的蛋白激酶1(MAPk1)、磷酸化细胞外信号调节激酶(PErk2)、磷酸肌醇3激酶(PI3k)、蛋白激酶b(Pkb/AkT)、丝氨酸/苏氨酸蛋白激酶(PAk)的表达。结果:与模型组比较,胃经穴组和对照点组大鼠胃溃疡指数值均显著降低(P<0.05),胃经穴组大鼠胃黏膜细胞MAPk1、PErk2、PI3k、AkT、PAk表达显著升高(P<0.05);与对照点组比较,胃经穴组大鼠胃溃疡指数和胃黏膜细胞MAPk1、PErk2、PI3k、AkT、PAk表达均显著升高(P<0.05)。结论:针刺可促进胃黏膜损伤修复,降低胃黏膜细胞增殖相关信号分子表达。; Objective: To study the expression of the proliferation signaling pathway on the gastric mucosa cells in rats with acute gastric mucosa injury treated by acupuncture.Methods: Rats were randomly divided into normal group, model group, stomach meridian group and control point group.The acute gastric mucosa injury model was established by the method of ethanol intragastric administration.The gastric mucosal injury indexes in rats were observed, the expression of the mitogenactivated protein kinase 1(MAPK1), phospho-extracelluar signal-regulated kinase2(pERK2), phosphoinositide3-kinase(PI3K), protein kinase B(PKB/Akt) and protein activated kinase(PAK) in gastric mucosal cell were tested with ELISA method.Results: Compared with model group, the gastric mucosa damage index in rats of stomach meridian and control point group were decreased(P<0.05), and the expression of the MAPK1, pERK2, PI3 K, Akt and PAK in gastric mucosa cells in stomach meridian group rats were decreased signfiicantly(P<0.05).Compared with control point group, the expression of the MAPK1, pERK2, PI3 K, Akt, PAK and gastric mucosa damage index in gastric mucosa cells in stomach meridian group rats were increased signfiicantly(P<0.05).Conclusion: The acupuncture could promote the gastric mucosa injury repair and decrease the expression of the proliferation signaling molecular in gastric mucosa cells.; 国家自然科学基金(No.30960484;No.81260556)~~
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/123765]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	王晨光,杨宗保,左彬荣,等. 针刺调节急性胃黏膜损伤大鼠胃黏膜细胞增殖信号通路相关分子的表达, Expression of the proliferation signaling pathway on the gastric mucosa cells in rats with acute gastric mucosa injury treated by acupuncture[J],2014.
APA	王晨光,杨宗保,左彬荣,谢宇峰,&刘琼.(2014).针刺调节急性胃黏膜损伤大鼠胃黏膜细胞增殖信号通路相关分子的表达..
MLA	王晨光,et al."针刺调节急性胃黏膜损伤大鼠胃黏膜细胞增殖信号通路相关分子的表达".(2014).

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