Designed composites for mimicking compressive mechanical properties of articular cartilage matrix

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	Designed composites for mimicking compressive mechanical properties of articular cartilage matrix
	Zhu, Youjia ; Wu, Hua ; Sun, Shaofa ; Zhou, Ting ; Wu, Jingjing ; Wan, Ying ; Wu H(吴华)
刊名	http://dx.doi.org/10.1016/j.jmbbm.2014.04.003
	2014
关键词	Cartilage Cell culture Collagen Composite materials Polycaprolactone Porosity Repair Strain
英文摘要	Collagen, chitosan-polycaprolactone (CH-PCL) copolymer with PCL content of around 40wt% and chondroitin sulfate (CS) were mixed together at various ratios to prepare collagen/CH-PCL/CS composites and the resulting composites were used to build stratified porous scaffolds that are potentially applicable for articular cartilage repair. The ternary composites were designed in such a way that collagen content in the scaffolds decreased from the top layer to the bottom layer while the content of CH-PCL and CS altered in a reversed trend in order to reach partial similarity to cartilage matrix in the composition of main components. Porous structures inside collagen/CH-PCL/CS scaffolds were constructed using a low-temperature deposition processing technique and graded average pore-size and porosity for the scaffolds were established. Such produced scaffolds were further crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide under optimized conditions, and the obtained scaffolds showed well-defined elastic compressive properties. Compressive modulus (E) and stress at 10% strain (σ10) of full scaffolds in wet state reached about 2.8MPa and 0.3MPa, respectively, and meanwhile, E and σ10 of layers inside hydrated scaffolds changed in a gradient-increased manner from the top layer to the bottom layer with significant differences between contiguous layers, which partially mimics compressive mechanical properties of cartilage matrix. In addition, in vitro culture of cell-scaffold constructs exhibited that scaffolds were able to well support the ingrowth and migration of seeded cells, and cells also showed relatively uniform distribution throughout the scaffolds. These results suggest that the presently developed collagen/CH-PCL/CS scaffolds have promising potential for applications in articular cartilage repair. ? 2014 Elsevier Ltd.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93727]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zhu, Youjia,Wu, Hua,Sun, Shaofa,et al. Designed composites for mimicking compressive mechanical properties of articular cartilage matrix[J]. http://dx.doi.org/10.1016/j.jmbbm.2014.04.003,2014.
APA	Zhu, Youjia.,Wu, Hua.,Sun, Shaofa.,Zhou, Ting.,Wu, Jingjing.,...&吴华.(2014).Designed composites for mimicking compressive mechanical properties of articular cartilage matrix.http://dx.doi.org/10.1016/j.jmbbm.2014.04.003.
MLA	Zhu, Youjia,et al."Designed composites for mimicking compressive mechanical properties of articular cartilage matrix".http://dx.doi.org/10.1016/j.jmbbm.2014.04.003 (2014).