Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells

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	Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells
	Zhou, Jieqiong ; Li, Weihua ; Xie, Qiang ; Hou, Yuxi ; Zhan, Shaopeng ; Yang, Xi ; Xu, Xiaofeng ; Cai, Jun ; Huang, Zhengrong ; Li WH(李卫华) ; Xie Q(谢强) ; Huang ZR(黄峥嵘)
刊名	http://dx.doi.org/10.1155/2014/376570
	2014
关键词	TREATMENT PANEL-III STATIN THERAPY COENZYME-Q CHOLESTEROL ATORVASTATIN METAANALYSIS PLASMA HYPERCHOLESTEROLEMIA EXPRESSION PREVENTION
英文摘要	Chinese National Science Foundation [81170090]; The aim of this study was to investigate the effects of simvastatin on insulin secretion in mouse MIN6 cells and the possible mechanism. MIN6 cells were, respectively, treated with 0 mu M, 2 mu M, 5 mu M, and 10 mu M simvastatin for 48 h. Radio immunoassay was performed to measure the effect of simvastatin on insulin secretion in MIN6 cells. Luciferase method was used to examine the content of ATP in MIN6 cells. Real-time PCR and western blotting were performed to measure the mRNA and protein levels of inward rectifier potassium channel 6.2 (Kir6.2), voltage-dependent calcium channel 1.2 (Ca(v)1.2), and glucose transporter-2 (GLUT2), respectively. ATP-sensitive potassium current and L-type calcium current were recorded by whole-cell patch-clamp technique. The results showed that high concentrations of simvastatin (5 mu M and 10 mu M) significantly reduced the synthesis and secretion of insulin compared to control groups in MIN6 cells (P < 0.05). ATP content in simvastatin-treated cells was lower than in control cells (P < 0.05). Compared with control group, the mRNA and protein expression of Kir6.2 increased with treatment of simvastatin (P < 0.05), and mRNA and protein expression of Ca(v)1.2 and GLUT2 decreased in response to simvastatin (P < 0.05). Moreover, simvastatin increased the ATP-sensitive potassium current and reduced the L-type calcium current. These results suggest that simvastatin inhibits the synthesis and secretion of insulin through a reduction in saccharometabolism in MIN6 cells.
语种	英语
出版者	HINDAWI PUBLISHING CORPORATION
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93695]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zhou, Jieqiong,Li, Weihua,Xie, Qiang,et al. Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells[J]. http://dx.doi.org/10.1155/2014/376570,2014.
APA	Zhou, Jieqiong.,Li, Weihua.,Xie, Qiang.,Hou, Yuxi.,Zhan, Shaopeng.,...&黄峥嵘.(2014).Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells.http://dx.doi.org/10.1155/2014/376570.
MLA	Zhou, Jieqiong,et al."Effects of Simvastatin on Glucose Metabolism in Mouse MIN6 Cells".http://dx.doi.org/10.1155/2014/376570 (2014).