Mycoepoxydiene inhibits activation of BV2 microglia stimulated by lipopolysaccharide through suppressing NF-kappa B, ERK 1/2 and toll-like receptor pathways

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	Mycoepoxydiene inhibits activation of BV2 microglia stimulated by lipopolysaccharide through suppressing NF-kappa B, ERK 1/2 and toll-like receptor pathways
	Su, Xinhui ; Chen, Qiang ; Chen, Wenbo ; Chen, Tenghui ; Li, Wenjiao ; Li, Yang ; Dou, Xiaofeng ; Zhang, Yafei ; Shen, Yuemao ; Wu, Hua ; Yu, Chundong ; Su XH(苏新辉) ; Shen YM(沈月毛) ; Wu H(吴华) ; Chen Q(陈强) ; Yu CD(俞春东)
刊名	http://dx.doi.org/10.1016/j.intimp.2014.01.004
	2014
关键词	PRO-INFLAMMATORY CYTOKINES NITRIC-OXIDE FUNGAL POLYKETIDE CELLS BETA BRAIN NEURODEGENERATION APOPTOSIS DISEASES PROTEIN
英文摘要	National Natural Science Foundation of China [81071182]; Medical Innovation Foundation of Fujian, China [2009-CXB-46]; Natural Science Foundation of Fujian Province of China [2010D015]; Youth Foundation of Health Department of Fujian Province [2010-2-84]; Mycoepoxydiene (MED) is a polyketide isolated from the marine fungal Diaporthe sp. HLY-1 associated with mangroves. Although MED has been shown to have various biological effects such as antimicrobial, anticancer, and anti-inflammatory activities, its activities and cellular mechanisms during microglial activation have yet to be elucidated. In the present study, we assessed the anti-inflammatory effect of MED on the production of inflammatory mediators in lipopolysaccharide (LPS)-stimulated murine BV2 microglia. MED significantly inhibited LPS-induced production of pro-inflammatory mediators such as tumor necrosis factor a (INF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), interferon-gamma (INF-gamma), and nitric oxide (NO), whereas it increased anti-inflammatory interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta 1) production in BV2 microglia in a concentration-dependent manner without causing cytotoxicity. Moreover, MED suppressed NF-kappa B activation by blocking IkappaB-alpha (I kappa B-alpha) degradation and inhibited the phosphorylation of ERK 1/2 and toll-like receptor 4 (TLR4) expression, but had no effect on the phosphorylation of JNK, and p38. Our results demonstrate that the inhibitory and promotion effect of MED on LPS-stimulated inflammatory mediators and anti-inflammatory factor production in BV2 microglia is associated with the suppression of the NF-kappa B, ERK 1/2 and TLR signaling pathways. Therefore, MED may have therapeutic potential for neurodegenerative diseases by inhibiting inflammatory mediators and enhancing anti-inflammatory factor production in activated microglia. (C) 2014 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93655]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Su, Xinhui,Chen, Qiang,Chen, Wenbo,et al. Mycoepoxydiene inhibits activation of BV2 microglia stimulated by lipopolysaccharide through suppressing NF-kappa B, ERK 1/2 and toll-like receptor pathways[J]. http://dx.doi.org/10.1016/j.intimp.2014.01.004,2014.
APA	Su, Xinhui.,Chen, Qiang.,Chen, Wenbo.,Chen, Tenghui.,Li, Wenjiao.,...&俞春东.(2014).Mycoepoxydiene inhibits activation of BV2 microglia stimulated by lipopolysaccharide through suppressing NF-kappa B, ERK 1/2 and toll-like receptor pathways.http://dx.doi.org/10.1016/j.intimp.2014.01.004.
MLA	Su, Xinhui,et al."Mycoepoxydiene inhibits activation of BV2 microglia stimulated by lipopolysaccharide through suppressing NF-kappa B, ERK 1/2 and toll-like receptor pathways".http://dx.doi.org/10.1016/j.intimp.2014.01.004 (2014).