Cholesterol modulates function of connexin 43 gap junction channel via PKC pathway in H9c2 cells

CORC > 厦门大学 > 医学院－已发表论文

	Cholesterol modulates function of connexin 43 gap junction channel via PKC pathway in H9c2 cells
	Zou, Jun ; Yue, Xiao-Yang ; Zheng, Sheng-Chao ; Zhang, Guangwei ; Chang, He ; Liao, Yan-Chun ; Zhang, Ye ; Xue, Mao-Qiang ; Qi, Zhi ; Zou J(邹军) ; Chang H(常贺) ; Zhang Y(张业) ; Xue MQ(薛茂强) ; Qi Z(戚智)
刊名	http://dx.doi.org/10.1016/j.bbamem.2014.04.016
	2014
关键词	PROTEIN-KINASE-C INTERCELLULAR COMMUNICATION MEMBRANE-STRUCTURE UP-REGULATION TUMOR-CELLS LIPID RAFTS FATTY-ACIDS PHOSPHORYLATION ACTIVATION CAVEOLIN-1
英文摘要	National Natural Science Foundation of China [31070741, 31270891, 81270294]; It has been shown that cholesterol modulates activity of protein kinase C (PKC), and PKC phosphorylates connexin 43 (Cx43) to regulate its function, respectively. However, it is not known whether cholesterol modulates function of Cx43 through regulating activity of PKC. In the present study, we demonstrated that cholesterol enrichment reduced the dye transfer ability of Cx43 in cultured H9c2 cells. Western blot analysis indicated that cholesterol enrichment enhanced the phosphorylated state of Cx43. Immunofluorescent images showed that cholesterol enrichment made the Cx43 distribution from condensed to diffused manner in the interface between the cells. In cholesterol enriched cells, PKC antagonists partially restored the dye transfer ability among the cells, downregulated the phosphoiylation of Cx43 and redistributed Cx43 from the diffused manner to the condensed manner in the cell interface. In addition, reduction of cholesterol level suppressed PKC activity to phosphorylate Cx43 and restored Cx43 function in PKC agonist-treated cells. Furthermore, we demonstrated that cholesterol enrichment upregulated the phosphorylated state of Cx43 at Ser368, while PKC antagonists reversed the effect. Taken together, cholesterol level in the cells plays important roles in regulating Cx43 function through activation of the PKC signaling pathway. (C) 2014 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93583]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zou, Jun,Yue, Xiao-Yang,Zheng, Sheng-Chao,et al. Cholesterol modulates function of connexin 43 gap junction channel via PKC pathway in H9c2 cells[J]. http://dx.doi.org/10.1016/j.bbamem.2014.04.016,2014.
APA	Zou, Jun.,Yue, Xiao-Yang.,Zheng, Sheng-Chao.,Zhang, Guangwei.,Chang, He.,...&戚智.(2014).Cholesterol modulates function of connexin 43 gap junction channel via PKC pathway in H9c2 cells.http://dx.doi.org/10.1016/j.bbamem.2014.04.016.
MLA	Zou, Jun,et al."Cholesterol modulates function of connexin 43 gap junction channel via PKC pathway in H9c2 cells".http://dx.doi.org/10.1016/j.bbamem.2014.04.016 (2014).