WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes

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	WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes
	Huang, Xin ; Ding, Lu ; Pan, Rui ; Ma, Peng-Fei ; Cheng, Pan-Pan ; Zhang, Chun-Hui ; Shen, Yu-Ting ; Xu, Lin ; Liu, Yu ; He, Xiao-Qin ; Qi, Zhong-Quan ; Wang, Hai-Long ; Xu L(许林) ; Qi ZQ(齐忠权) ; Wang HL(王海龙)
刊名	http://dx.doi.org/10.1007/s00418-012-1051-z
	2013
关键词	ARP2/3 COMPLEX CORTICAL POLARITY CELL-DIVISION ACTIN MATURATION FERTILIZATION DYNAMICS EGGS MICROFILAMENTS CENTROSOME
英文摘要	National Natural Science Foundation of China [31201695]; Major State Scientific Research Program of China [2012CBA01303]; WASP homolog associated with actin, membranes and microtubules (WHAMM) is a newly discovered nucleation-promoting factor that links actin and microtubule cytoskeleton and regulates transport from the endoplasmic reticulum to the Golgi apparatus. However, knowledge of WHAMM is limited to interphase somatic cells. In this study, we examined its localization and function in mouse oocytes during meiosis. Immunostaining showed that in the germinal vesicle (GV) stage, there was no WHAMM signal; after meiosis resumption, WHAMM was associated with the spindle at prometaphase I (Pro MI), metaphase I (MI), telophase I (TI) and metaphase II (MII) stages. Nocodazole and taxol treatments showed that WHAMM was localized around the MI spindle. Depletion of WHAMM by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in failure of spindle migration, disruption of asymmetric cytokinesis and a decrease in the first polar body extrusion rate during meiotic maturation. Moreover, actin cap formation was also disrupted after WHAMM depletion, confirming the failure of spindle migration. Taken together, our data suggest that WHAMM is required for peripheral spindle migration and asymmetric cytokinesis during mouse oocyte maturation.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93568]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Huang, Xin,Ding, Lu,Pan, Rui,et al. WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes[J]. http://dx.doi.org/10.1007/s00418-012-1051-z,2013.
APA	Huang, Xin.,Ding, Lu.,Pan, Rui.,Ma, Peng-Fei.,Cheng, Pan-Pan.,...&王海龙.(2013).WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes.http://dx.doi.org/10.1007/s00418-012-1051-z.
MLA	Huang, Xin,et al."WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes".http://dx.doi.org/10.1007/s00418-012-1051-z (2013).