Effects of small interfering RNA targeting sphingosine kinase-1 gene on the animal model of Alzheimer's disease

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	Effects of small interfering RNA targeting sphingosine kinase-1 gene on the animal model of Alzheimer's disease
	Zhang, Yuan ; Yu, Qian ; Lai, Tian-bao ; Yang, Yang ; Li, Gang ; Sun, Sheng-gang ; Yang Y(杨扬)
刊名	http://dx.doi.org/10.1007/s11596-013-1136-5
	2013
关键词	AMYLOID-BETA-PEPTIDE PRECURSOR PROTEIN CERAMIDE NEURONS SPHINGOSINE-1-PHOSPHATE BRAIN INVOLVEMENT SECRETION SURVIVAL ROLES
英文摘要	National Natural Science Foundation of China [81070879]; Alzheimer's disease (AD) is an age-related, progressive neurodegenerative disorder that occurs gradually and results in memory, behavior, and personality changes. Abnormal sphingolipid metabolism was reported in AD previously. This study aimed to investigate whether sphK1 could exacerbate the accumulation of amyloid protein (A beta) and sharpen the learning and memory ability of the animal model of AD using siRNA interference. An adenovirus vector expressing small interfering RNA (siRNA) against the sphK1 gene (sphK1-siRNA) was designed, and the effects of sphK1-siRNA on the APP/PS1 mouse four weeks after treatment with sphK1-siRNA hippocampal injection were examined. SphK1 protein expression was confirmed by using Western blotting and ceramide content coupled with S1P secretion was evaluated by enzyme-linked immunosorbent assay (ELISA). A beta load was detected by immunohistochemical staining and ELISA. Morris water maze was adopted to test the learning and memory ability of the APP/PS1 mice. A significant difference in the expression of sphK1 protein and mRNA was observed between the siRNA group and the control group. A beta load in transfected mice was accelerated in vivo, with significant aggravation of the learning and memory ability. The sphK1 gene modulation in the A beta load and the learning and memory ability in the animal model of AD may be important for the treatment of AD.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93538]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zhang, Yuan,Yu, Qian,Lai, Tian-bao,et al. Effects of small interfering RNA targeting sphingosine kinase-1 gene on the animal model of Alzheimer's disease[J]. http://dx.doi.org/10.1007/s11596-013-1136-5,2013.
APA	Zhang, Yuan.,Yu, Qian.,Lai, Tian-bao.,Yang, Yang.,Li, Gang.,...&杨扬.(2013).Effects of small interfering RNA targeting sphingosine kinase-1 gene on the animal model of Alzheimer's disease.http://dx.doi.org/10.1007/s11596-013-1136-5.
MLA	Zhang, Yuan,et al."Effects of small interfering RNA targeting sphingosine kinase-1 gene on the animal model of Alzheimer's disease".http://dx.doi.org/10.1007/s11596-013-1136-5 (2013).