Twist2 promotes ovarian cancer cell survival through activation of Akt | |
Mao, Yubin ; Xu, Jinfei ; Song, Gang ; Zhang, Nini ; Yin, Hao ; Mao YB(毛宇彬) ; Song G(宋刚) ; Zhang NN(张妮妮) | |
刊名 | http://dx.doi.org/10.3892/ol.2013.1316
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2013 | |
关键词 | EPITHELIAL-MESENCHYMAL TRANSITION UP-REGULATION CARCINOMA CELLS HYPOXIA HIF-1-ALPHA EXPRESSION PROGRESSION METASTASIS PATHWAY SNAIL |
英文摘要 | National Natural Science Foundation of China [30872515, 31071187, 81272721]; Fundamental Research Funds for the Central Universities of China [2011121062]; Natural Science Foundation of Fujian Province of China [2012J01417]; Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is an important prognostic factor in ovarian carcinoma. Hypoxia contributes to tumor progression and is involved in the epithelial-mesenchymal transition (EMT). Twist2 is an EMT regulator, however, it remains poorly understood in ovarian carcinoma. The present study evaluated the expression of HIF-1 alpha and Twist2 and further investigated whether Twist2 is involved in hypoxia-induced apoptosis in ovarian cancer. A series of matched paraffin-embedded tissue sections from human primary ovarian cancer and normal ovarian tissues were examined through immunohistochemical analysis, a Twist2-overexpressing stable ovarian cancer cell line was established and deferoxamine (DFO) was introduced to simulate hypoxic conditions. DFO-induced apoptosis was examined by fluorescence microscopy, MTT assays and flow cytometry. In addition, a western blot analysis was performed to examine the molecular mechanism(s) of action. Twist2 increased in epithelial ovarian cancers associated with HIF-1 alpha expression. The acquired expression of Twist2 was able to promote the survival of ovarian cancer cells through Akt phosphorylation under DFO-induced hypoxic stress. The results suggest that Twist2 activates the PI-3K-Akt pathway to protect cells from apoptosis in a hypoxic environment. Moreover, Twist2 may be involved in the HIF-1 alpha signaling pathway in ovarian cancer. |
语种 | 英语 |
出版者 | SPANDIDOS PUBL LTD |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/93529] ![]() |
专题 | 医学院-已发表论文 |
推荐引用方式 GB/T 7714 | Mao, Yubin,Xu, Jinfei,Song, Gang,et al. Twist2 promotes ovarian cancer cell survival through activation of Akt[J]. http://dx.doi.org/10.3892/ol.2013.1316,2013. |
APA | Mao, Yubin.,Xu, Jinfei.,Song, Gang.,Zhang, Nini.,Yin, Hao.,...&张妮妮.(2013).Twist2 promotes ovarian cancer cell survival through activation of Akt.http://dx.doi.org/10.3892/ol.2013.1316. |
MLA | Mao, Yubin,et al."Twist2 promotes ovarian cancer cell survival through activation of Akt".http://dx.doi.org/10.3892/ol.2013.1316 (2013). |
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