Twist2 promotes ovarian cancer cell survival through activation of Akt

CORC > 厦门大学 > 医学院－已发表论文

	Twist2 promotes ovarian cancer cell survival through activation of Akt
	Mao, Yubin ; Xu, Jinfei ; Song, Gang ; Zhang, Nini ; Yin, Hao ; Mao YB(毛宇彬) ; Song G(宋刚) ; Zhang NN(张妮妮)
刊名	http://dx.doi.org/10.3892/ol.2013.1316
	2013
关键词	EPITHELIAL-MESENCHYMAL TRANSITION UP-REGULATION CARCINOMA CELLS HYPOXIA HIF-1-ALPHA EXPRESSION PROGRESSION METASTASIS PATHWAY SNAIL
英文摘要	National Natural Science Foundation of China [30872515, 31071187, 81272721]; Fundamental Research Funds for the Central Universities of China [2011121062]; Natural Science Foundation of Fujian Province of China [2012J01417]; Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is an important prognostic factor in ovarian carcinoma. Hypoxia contributes to tumor progression and is involved in the epithelial-mesenchymal transition (EMT). Twist2 is an EMT regulator, however, it remains poorly understood in ovarian carcinoma. The present study evaluated the expression of HIF-1 alpha and Twist2 and further investigated whether Twist2 is involved in hypoxia-induced apoptosis in ovarian cancer. A series of matched paraffin-embedded tissue sections from human primary ovarian cancer and normal ovarian tissues were examined through immunohistochemical analysis, a Twist2-overexpressing stable ovarian cancer cell line was established and deferoxamine (DFO) was introduced to simulate hypoxic conditions. DFO-induced apoptosis was examined by fluorescence microscopy, MTT assays and flow cytometry. In addition, a western blot analysis was performed to examine the molecular mechanism(s) of action. Twist2 increased in epithelial ovarian cancers associated with HIF-1 alpha expression. The acquired expression of Twist2 was able to promote the survival of ovarian cancer cells through Akt phosphorylation under DFO-induced hypoxic stress. The results suggest that Twist2 activates the PI-3K-Akt pathway to protect cells from apoptosis in a hypoxic environment. Moreover, Twist2 may be involved in the HIF-1 alpha signaling pathway in ovarian cancer.
语种	英语
出版者	SPANDIDOS PUBL LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93529]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Mao, Yubin,Xu, Jinfei,Song, Gang,et al. Twist2 promotes ovarian cancer cell survival through activation of Akt[J]. http://dx.doi.org/10.3892/ol.2013.1316,2013.
APA	Mao, Yubin.,Xu, Jinfei.,Song, Gang.,Zhang, Nini.,Yin, Hao.,...&张妮妮.(2013).Twist2 promotes ovarian cancer cell survival through activation of Akt.http://dx.doi.org/10.3892/ol.2013.1316.
MLA	Mao, Yubin,et al."Twist2 promotes ovarian cancer cell survival through activation of Akt".http://dx.doi.org/10.3892/ol.2013.1316 (2013).