Antinociceptive effect of berberine on visceral hypersensitivity in rats

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	Antinociceptive effect of berberine on visceral hypersensitivity in rats
	Tang, Qing-Lin ; Lai, Min-Ling ; Zhong, Yuan-Fu ; Wang, Ai-Min ; Su, Jun-Kai ; Zhang, Ming-Qing ; Tang QL(唐庆林) ; Su JK(苏军凯) ; Zhang MQ(张鸣青)
刊名	http://dx.doi.org/10.3748/wjg.v19.i28.4582
	2013
关键词	IRRITABLE-BOWEL-SYNDROME NITRIC-OXIDE SYNTHASE IN-VITRO ANTIMICROBIAL ACTIVITY HEALTHY-SUBJECTS CELLS EXPRESSION COLITIS MODEL COMBINATION
英文摘要	AIM: To assess the protective effect of berberine administration and the role of nitric oxide (NO) in visceral hypersensitivity. METHODS: Fifty male Sprague-Dawley rats were randomly assigned to five groups. An inflammatory bowel disease model was induced in rats by intracolonic instillation of 1 mL 4% acetic acid at 8 cm proximal to the anus for 30 s and restraint stress. After subsidence of inflammation on day 7 of the experiment, the rats were subjected to rectal distension, performed by a balloon (6-Fr, 2 mm external diameter, disposable silicon balloon-urethral catheter for pediatric use) which was rapidly inflated with increasing volumes of prewarmed (37 degrees C) water (0.1, 0.2, 0.3, 0.4, 0.6, 0.8 and 1 mL) for 30 s at four-minute intervals, and then the abdominal withdrawal reflex (AWR) and the level of fecal output were measured, respectively. AWR scores either 0, 1, 2, 3 or 4 were obtained by blinded observers. Rats had been pretreated with berberine or aminoguanidine (NO synthetase inhibitor) or berberine + aminoguanidine before measurement. RESULTS: The rats in the placebo group showed a hypersensitive response to rectal distension (2.69 +/- 0.08 vs 1.52 +/- 0.08, P = 0.000) and defecated more frequently than those in the control group (5.0 +/- 0.16 vs 0.44 +/- 0.16, P = 0.000). Comparing the berberine with placebo group, the AWR scores were reduced for all distension volumes and were significant at 0.2-1 mL (1.90 +/- 0.08 vs 2.69 +/- 0.08, P = 0.000), while the numbers of hard pellets, soft pellets, formless stools, and total fecal output in the placebo group were significantly larger than in the berberine group (5.0 +/- 0.16 vs 2.56 +/- 0.16, P = 0.000). Administration of aminoguanidine or berberine + aminoguanidine before VH score measurement reversed the antinociceptive effect of berberine (2.52 +/- 0.08 vs 1.90 +/- 0.08, P = 0.000; 2.50 +/- 0.08 vs 1.90 +/- 0.08, P = 0.000). The numbers of hard pellets, soft pellets, formless stool, and total of fecal output in aminoguanidine group were significantly larger than the corresponding values in control group, berberine group, and berberine + aminoguanidine group (4.81 +/- 0.16 vs 0.44 +/- 0.16, P = 0.000; 4.81 +/- 0.16 vs 2.56 +/- 0.16, P = 0.000; 4.81 +/- 0.16 vs 3.75 +/- 0.16, P = 0.000). The berberine and berberine + aminoguanidine groups showed reduced defecation, but aminoguanidine alone did not reduce defecation (2.56 +/- 0.16 vs 4.81 +/- 0.16, P = 0.000; 3.75 +/- 0.16 vs 4.81 +/- 0.16, P = 0.000). CONCLUSION: Berberine had an antinociceptive effect on visceral hypersensitivity, and NO might play a role in this effect. (C) 2013 Baishideng. All rights reserved.
语种	英语
出版者	BAISHIDENG PUBL GRP CO LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93518]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Tang, Qing-Lin,Lai, Min-Ling,Zhong, Yuan-Fu,et al. Antinociceptive effect of berberine on visceral hypersensitivity in rats[J]. http://dx.doi.org/10.3748/wjg.v19.i28.4582,2013.
APA	Tang, Qing-Lin.,Lai, Min-Ling.,Zhong, Yuan-Fu.,Wang, Ai-Min.,Su, Jun-Kai.,...&张鸣青.(2013).Antinociceptive effect of berberine on visceral hypersensitivity in rats.http://dx.doi.org/10.3748/wjg.v19.i28.4582.
MLA	Tang, Qing-Lin,et al."Antinociceptive effect of berberine on visceral hypersensitivity in rats".http://dx.doi.org/10.3748/wjg.v19.i28.4582 (2013).