Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model | |
Xiao, Chuan-Xing ; Wang, Huan-Huan ; Shi, Ying ; Li, Ping ; Liu, Yun-Peng ; Ren, Jian-Lin ; Guleng, Bayasi ; Xiao CX(肖传兴) ; Wang HH(王欢欢) ; Ren JL(任建林) ; 巴亚斯古楞 | |
刊名 | http://dx.doi.org/10.1371/journal.pone.0073666
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2013 | |
关键词 | TUMOR ANGIOGENESIS INTESTINAL INFLAMMATION DENDRITIC CELLS MYELOID CELLS PROGENITOR CELLS STEM-CELL T-CELLS GROWTH TOLERANCE |
英文摘要 | National Natural Science Foundation of China [81072013, 91229201]; Fundamental Research Funds for the Central Universities in China [2010111082]; Foundations of Health Bureau of Xiamen in China [3502Z200940010]; Inflammatory bowel disease (IBD) can lead to an increased risk of developing colorectal cancer (CRC). The aim of this study was to establish a model for combined bone marrow transplantation (BMT) and colitis-associated colorectal cancer (CAC) and to define the contribution of BM-derived cells during the inflammation associated with carcinogenesis. We established a model for BMT using green fluorescent protein (GFP) transgenic mice, followed by AOM/DSS-induced CAC, and performed confocal microscopy analysis on in vivo living tissue and frozen tumor sections. Our imaging analyses showed that GFP-positive cells extensively infiltrated the tumor stroma and that some WGA and GFP or CD31 and GFP double-positive cells were observed in the lining of tumor vessels. Flow cytometry analysis of the tumor-infiltrating cells showed that the GFP-positive CD11c+ DCs cells were one-third of the GFP+/CD11C-cells, and that half of these DCs (0.96% vs 1.02%) were GFP-positive BM-derived cells. The majority of CD4(+) T cells were GFP-negative (12.02% vs 1.9%), and we discovered a novel CD4(+) CD11c(+) DC subset (0.34% vs 1.64%). In conclusion, we defined the distribution of BM-derived endothelial cells, CD11c(+) DCs and CD4(+) T cells in tumors. This model might be useful for elucidating the diverse BM-derived cell types and functions during the progression of colitis-associated colorectal cancer. |
语种 | 英语 |
出版者 | PUBLIC LIBRARY SCIENCE |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/93491] ![]() |
专题 | 医学院-已发表论文 |
推荐引用方式 GB/T 7714 | Xiao, Chuan-Xing,Wang, Huan-Huan,Shi, Ying,et al. Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model[J]. http://dx.doi.org/10.1371/journal.pone.0073666,2013. |
APA | Xiao, Chuan-Xing.,Wang, Huan-Huan.,Shi, Ying.,Li, Ping.,Liu, Yun-Peng.,...&巴亚斯古楞.(2013).Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model.http://dx.doi.org/10.1371/journal.pone.0073666. |
MLA | Xiao, Chuan-Xing,et al."Distribution of Bone-Marrow-Derived Endothelial and Immune Cells in a Murine Colitis-Associated Colorectal Cancer Model".http://dx.doi.org/10.1371/journal.pone.0073666 (2013). |
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