Ether a go-go 1 Silencing in Combination with TRAIL Overexpression Has Synergistic Antitumor Effects on Osteosarcoma

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	Ether a go-go 1 Silencing in Combination with TRAIL Overexpression Has Synergistic Antitumor Effects on Osteosarcoma
	Wu, Jin ; Zeng, Tian ; Wu, Xinyu ; Gao, Quan ; Zhai, Wenliang ; Ding, Zhenqi ; Wu XY(吴欣宇)
刊名	http://dx.doi.org/10.1089/cbr.2012.1283
	2013
关键词	POTASSIUM CHANNEL ETHER EAG K+ CHANNELS POLY(ADP-RIBOSE) POLYMERASE PROGNOSTIC-FACTORS INDUCED APOPTOSIS EWINGS-SARCOMA CELL-LINES IN-VITRO EXPRESSION LIGAND
英文摘要	Fujian Natural Science Foundation, China [C0710048]; Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been utilized for cancer therapy, but the resistance of cancer cells to TRAIL remains an obstacle. Ether a go-go 1 (Eag1) channel is overexpressed in a variety of cancers and implicated in tumor progression. However, the therapeutic potential of Eag1 in osteosarcoma remains elusive. In this study, we generated CRAd5.TRAIL/siEag1 adenoviral vector that permitted simultaneous knockdown of Eag1 and overexpression of TRAIL and investigated its antitumor effects on human osteosarcoma MG-63 cells. Our results showed that CRAd5.TRAIL/siEag1 induced growth arrest and apoptosis of MG-63 cells in a more efficient manner than CRAd5.TRAIL or CRAd5.siEag1, and had no effect on human osteoblastic hFOB1.19 cells. Furthermore, treatment of an osteosarcoma xenograft model with CRAd5.TRAIL/siEag1 resulted in significant tumor regression and cancer cell apoptosis, compared with treatment with CRAd5. TRAIL or CRAd5.siEag1. Taken together, our results demonstrate that CRAd5.TRAIL/siEag1 may represent an effective strategy for osteosarcoma gene therapy due to the synergistic antitumor effects of Eag1 knockdown and TRAIL overexpression.
语种	英语
出版者	MARY ANN LIEBERT INC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93404]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Wu, Jin,Zeng, Tian,Wu, Xinyu,et al. Ether a go-go 1 Silencing in Combination with TRAIL Overexpression Has Synergistic Antitumor Effects on Osteosarcoma[J]. http://dx.doi.org/10.1089/cbr.2012.1283,2013.
APA	Wu, Jin.,Zeng, Tian.,Wu, Xinyu.,Gao, Quan.,Zhai, Wenliang.,...&吴欣宇.(2013).Ether a go-go 1 Silencing in Combination with TRAIL Overexpression Has Synergistic Antitumor Effects on Osteosarcoma.http://dx.doi.org/10.1089/cbr.2012.1283.
MLA	Wu, Jin,et al."Ether a go-go 1 Silencing in Combination with TRAIL Overexpression Has Synergistic Antitumor Effects on Osteosarcoma".http://dx.doi.org/10.1089/cbr.2012.1283 (2013).