Short Hairpin RNA (shRNA) Ether a go-go 1 (Eag1) Inhibition of Human Osteosarcoma Angiogenesis via VEGF/PI3K/AKT Signaling

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	Short Hairpin RNA (shRNA) Ether a go-go 1 (Eag1) Inhibition of Human Osteosarcoma Angiogenesis via VEGF/PI3K/AKT Signaling
	Wu, Jin ; Wu, Xinyu ; Zhong, Daixing ; Zhai, Wenliang ; Ding, Zhenqi ; Zhou, Yong ; Wu J(吴进) ; Zhai WL(翟文亮) ; Ding ZQ(丁真奇) ; Wu XY(吴欣宇)
刊名	http://dx.doi.org/10.3390/ijms131012573
	2012-10
关键词	ENDOTHELIAL GROWTH-FACTOR POTASSIUM CHANNELS EWINGS-SARCOMA CANCER TUMORS VEGF METASTASIS THERAPY BIOLOGY EXPRESSION
英文摘要	Fujian Natural Science Foundation, China [C0710048]; Ether a go-go 1 (Eag1) channel is overexpressed in a variety of cancers but the therapeutic potential of Eag1 in osteosarcoma remains elusive. In this study, we constructed an Ad5-Eag1-shRNA vector and evaluated its efficiency for Eag1 knockdown and its effects on osteosarcoma. Our results showed that Ad5-Eag1-shRNA had high interference efficiency of Eag1 expression and suppressed osteosarcoma growth both in vitro and in vivo. To explore the molecular mechanism underlying tumor growth inhibition induced by Eag1 silencing, the intratumoral microvessel density (MVD) was assessed by CD31 staining and the expression of vascular endothelial growth factor (VEGF) was detected by Western blot analysis. We found that Eag1 silencing led to decreased angiogenesis and VEGF expression in the xenograft model of osteosarcoma. Finally, we detected a time-dependent decrease in VEGF expression and considerably reduced phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) activation in osteosarcoma cells treated by Eag1 shRNA. Taken together, our results suggest that Eag1 silencing inhibits tumor growth and angiogenesis in osteosarcoma via the down regulation of VEGF/PI3K/AKT signaling.
语种	英语
出版者	INT J MOL SCI
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93358]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Wu, Jin,Wu, Xinyu,Zhong, Daixing,et al. Short Hairpin RNA (shRNA) Ether a go-go 1 (Eag1) Inhibition of Human Osteosarcoma Angiogenesis via VEGF/PI3K/AKT Signaling[J]. http://dx.doi.org/10.3390/ijms131012573,2012.
APA	Wu, Jin.,Wu, Xinyu.,Zhong, Daixing.,Zhai, Wenliang.,Ding, Zhenqi.,...&吴欣宇.(2012).Short Hairpin RNA (shRNA) Ether a go-go 1 (Eag1) Inhibition of Human Osteosarcoma Angiogenesis via VEGF/PI3K/AKT Signaling.http://dx.doi.org/10.3390/ijms131012573.
MLA	Wu, Jin,et al."Short Hairpin RNA (shRNA) Ether a go-go 1 (Eag1) Inhibition of Human Osteosarcoma Angiogenesis via VEGF/PI3K/AKT Signaling".http://dx.doi.org/10.3390/ijms131012573 (2012).