Nicotine Stimulated Dendritic Cells Could Achieve Anti-Tumor Effects in Mouse Lung and Liver Cancer

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	Nicotine Stimulated Dendritic Cells Could Achieve Anti-Tumor Effects in Mouse Lung and Liver Cancer
	Gao, Feng Guang ; Li, Hai Tao ; Li, Zhi Jing ; Gu, Jian Ren ; Gao FG(高丰光)
刊名	http://dx.doi.org/10.1007/s10875-010-9459-5
	2011-01
关键词	ACETYLCHOLINE-RECEPTOR VACCINATION MATURE PROLIFERATION INFLAMMATION SUPPRESSION ACTIVATION EXPRESSION RESPONSES MELANOMA
英文摘要	Natural Science Foundation of Fujian Province of China [2008J0112]; National Laboratory for Oncogenes and Related Genes of China [90-08-02]; Our previous studies have revealed that nicotine-treated immature dendritic cells (imDCs) have anti-tumor effects in murine lymphoma models. The present study is to explore the preventive and therapeutic anti-tumor effects of nicotine-treated imDCs in murine lung and liver cancer. To address this objection, bone marrow-derived imDCs were firstly stimulated by nicotine in vitro and the expressions of CD80, CD86, CD40, CD11b, MHC class I and II were determined by flow cytometry. Then, DCs-dependent tumor-lysate-specific T cell proliferation, IL-12(p40+p70) secretion were determined by BrdU cell proliferation assay and enzyme-linked immunosorbent assay, respectively. The anti-tumor effects of such imDCs were further explored by intraperitoneal transfer against tumor challenge or implantation. By using kinase inhibitors, the mechanism of nicotine upregulating CD80 was finally explored by flow cytometry. The results showed that: firstly, nicotine could upregulate the expressions of CD80, CD86, CD40,CD11b, MHC class I and II molecules in imDCs. Secondly, nicotine could promote imDCs-dependent T cell priming and IL-12 secretion. Most importantly, systemic transfer of ex vivo nicotine-stimulated imDCs, which enhanced CD80 expression through PI3K activation, could reveal preventive and effectively therapeutic effects on tumor development. Ex vivo nicotine stimulation can significantly improve imDCs efficacy for adaptive therapy of cancer. Nicotine-treated imDCs might be considered as a potential candidate for therapeutic tumor immunotherapy for lung and liver cancer.
语种	英语
出版者	J CLIN IMMUNOL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93326]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Gao, Feng Guang,Li, Hai Tao,Li, Zhi Jing,et al. Nicotine Stimulated Dendritic Cells Could Achieve Anti-Tumor Effects in Mouse Lung and Liver Cancer[J]. http://dx.doi.org/10.1007/s10875-010-9459-5,2011.
APA	Gao, Feng Guang,Li, Hai Tao,Li, Zhi Jing,Gu, Jian Ren,&高丰光.(2011).Nicotine Stimulated Dendritic Cells Could Achieve Anti-Tumor Effects in Mouse Lung and Liver Cancer.http://dx.doi.org/10.1007/s10875-010-9459-5.
MLA	Gao, Feng Guang,et al."Nicotine Stimulated Dendritic Cells Could Achieve Anti-Tumor Effects in Mouse Lung and Liver Cancer".http://dx.doi.org/10.1007/s10875-010-9459-5 (2011).