JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation

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	JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation
	Huang, Xin ; Tong, Jing-Shan ; Wang, Zhen-Bo ; Yang, Cai-Rong ; Qi, Shu-Tao ; Guo, Lei ; Ouyang, Ying-Chun ; Quan, Song ; Sun, Qing-Yuan ; Qi, Zhong-Quan ; Huang, Ru-Xin ; Wang, Hai-Long ; Qi ZQ(齐忠权) ; Wang HL(王海龙)
刊名	http://dx.doi.org/10.1017/S1431927610094456
	2011-04
关键词	N-TERMINAL KINASE ACTIVATED PROTEIN-KINASE POLO-LIKE KINASE-1 CELL-CYCLE DNA-DAMAGE CHROMOSOME ALIGNMENT CANCER CELLS APOPTOSIS CHECKPOINT PROLIFERATION
英文摘要	National Natural Science Foundation of China [30930065]; National Basic Research Program [2001CB944500]; It is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. In this study, we found that no specific JNK2 signal was detected in germinal vesicle stage. JNK2 was associated with the spindles especially the spindle poles and cytoplasmic microtubule organizing centers at prometaphase I, metaphase I, and metaphase II stages. JNK2 became diffusely distributed and associated with the midbody at telophase I stage. Injection of myc-tagged JNK2 alpha 1 mRNA into oocytes also revealed its localization on spindle poles. The association of JNK2 with spindle poles was further confirmed by colocalization with the centrosomal proteins, gamma-tubulin and Plk1. Nocodazole treatment showed that JNK2 may interact with Plk1 to regulate the spindle assembly. Then we investigated the possible function of JNK2 by JNK2 antibody microinjection and JNK specific inhibitor SP600125 treatment. These two manipulations caused abnormal spindle formation and decreased the rate of first polar body (PB1) extrusion. In addition, inhibition of JNK2 resulted in impaired localization of Plk1. Taken together, our results suggest that JNK2 plays an important role in spindle assembly and PB1 extrusion during mouse oocyte meiotic maturation.
语种	英语
出版者	MICROSC MICROANAL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93313]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Huang, Xin,Tong, Jing-Shan,Wang, Zhen-Bo,et al. JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation[J]. http://dx.doi.org/10.1017/S1431927610094456,2011.
APA	Huang, Xin.,Tong, Jing-Shan.,Wang, Zhen-Bo.,Yang, Cai-Rong.,Qi, Shu-Tao.,...&王海龙.(2011).JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation.http://dx.doi.org/10.1017/S1431927610094456.
MLA	Huang, Xin,et al."JNK2 Participates in Spindle Assembly during Mouse Oocyte Meiotic Maturation".http://dx.doi.org/10.1017/S1431927610094456 (2011).